Lymphomas with follicular and monocytoid B-cell components

Evidence for a common clonal origin from follicle center cells

Abdou Abou-Elella, Michael T. Shafer, Xiao Y. Wan, Milind Velanker, Dennis D. Weisenburger, Bharat N. Nathwani, Randy D. Gascoyne, Timothy Charles Greiner, Wing C. Chan

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

We investigated the clonal relationship between follicular center cell and monocytoid B-cell components of non-Hodgkin lymphoma by isolating the components and comparing the nucleotide sequences of the complementarity-determining region (CDR)3 of the rearranged immunoglobulin heavy chain (IgH) gene. Paraffin blocks from 4 cases with amplifiable DNA using the polymerase chain reaction (PCR) were identified. Multiple representative cell clusters of the 2 components were obtained by microdissection, and the IgH CDR3 was amplified using a seminested PCR. Most of the PCR products obtained from both tumor components in each case had identical lengths when analyzed with polyacrylamide gel electrophoresis (PAGE) and identical migratory patterns on denaturing gradient gel electrophoresis (DGGE). These findings indicate sequence identity of the IgH CDR3 of both tumor components. Sequence analysis showed that point mutations were responsible for bands from the same case that had nonidentical migratory patterns by DGGE. The components in each of the 4 cases studied have the same clonal origin. Intraclonal sequence variations in the IgH gene were observed in 2 cases, consistent with the presence of continued somatic hypermutation after establishment of the clone. The expression of CD10 and bcl-2, as well as the detection of bcl-2 rearrangements in 2 cases, indicate that these lymphomas are of follicular center cell origin.

Original languageEnglish (US)
Pages (from-to)516-522
Number of pages7
JournalAmerican journal of clinical pathology
Volume114
Issue number4
DOIs
StatePublished - Oct 1 2000

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Follicular Lymphoma
Cellular Structures
Immunoglobulin Heavy Chain Genes
Immunoglobulin Heavy Chains
Denaturing Gradient Gel Electrophoresis
B-Lymphocytes
Polymerase Chain Reaction
Complementarity Determining Regions
Microdissection
DNA-Directed DNA Polymerase
Point Mutation
Paraffin
Non-Hodgkin's Lymphoma
Sequence Analysis
Polyacrylamide Gel Electrophoresis
Neoplasms
Clone Cells

Keywords

  • Clonal relationship
  • Follicular lymphoma
  • IgH rearrangement
  • Marginal zone lymphoma
  • Monocytoid B-cell lymphoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Abou-Elella, A., Shafer, M. T., Wan, X. Y., Velanker, M., Weisenburger, D. D., Nathwani, B. N., ... Chan, W. C. (2000). Lymphomas with follicular and monocytoid B-cell components: Evidence for a common clonal origin from follicle center cells. American journal of clinical pathology, 114(4), 516-522. https://doi.org/10.1309/X559-FDJB-LJGD-YG7E

Lymphomas with follicular and monocytoid B-cell components : Evidence for a common clonal origin from follicle center cells. / Abou-Elella, Abdou; Shafer, Michael T.; Wan, Xiao Y.; Velanker, Milind; Weisenburger, Dennis D.; Nathwani, Bharat N.; Gascoyne, Randy D.; Greiner, Timothy Charles; Chan, Wing C.

In: American journal of clinical pathology, Vol. 114, No. 4, 01.10.2000, p. 516-522.

Research output: Contribution to journalArticle

Abou-Elella, A, Shafer, MT, Wan, XY, Velanker, M, Weisenburger, DD, Nathwani, BN, Gascoyne, RD, Greiner, TC & Chan, WC 2000, 'Lymphomas with follicular and monocytoid B-cell components: Evidence for a common clonal origin from follicle center cells', American journal of clinical pathology, vol. 114, no. 4, pp. 516-522. https://doi.org/10.1309/X559-FDJB-LJGD-YG7E
Abou-Elella, Abdou ; Shafer, Michael T. ; Wan, Xiao Y. ; Velanker, Milind ; Weisenburger, Dennis D. ; Nathwani, Bharat N. ; Gascoyne, Randy D. ; Greiner, Timothy Charles ; Chan, Wing C. / Lymphomas with follicular and monocytoid B-cell components : Evidence for a common clonal origin from follicle center cells. In: American journal of clinical pathology. 2000 ; Vol. 114, No. 4. pp. 516-522.
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AU - Velanker, Milind

AU - Weisenburger, Dennis D.

AU - Nathwani, Bharat N.

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AU - Greiner, Timothy Charles

AU - Chan, Wing C.

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