To identify lymphoid cells bearing receptors for both sheep erythrocytes (sE) and complement (C′), a combined rosette assay was developed using uncoated sE and chicken erythrocytes coated with antibody and complement (cEAC′). Cells with both receptors were present in low percentages in the blood of normal individuals. Tissues and/or blood from 543 patients with various diseases were studied using the combined rosette assay. Large numbers of malignant cells were found to carry receptors for both C′ and sE in four patients with lymphoproliferative malignancies. Two were children with lymphoma who had mediastinal masses. The other two were adults with chronic lymphocytic leukemia; their cells also carried surface immunoglobulin and Fc receptors. In these four patients double or multiple receptor lymphocytes may represent a clonal proliferation of subsets of lymphocytes present in small numbers under normal conditions. An increase in double receptor lymphocytes was rare in the non-neoplastic conditions studied and seen only in children. Double receptor lymphocytes may represent an immature or "stem" cell lymphocyte subset.
ASJC Scopus subject areas
- Immunology and Allergy
- Pathology and Forensic Medicine