Luminal short-chain fatty acids and postresection intestinal adaptation

Jon S Thompson, Eamonn M. Quigley, J. M. Palmer, William W. West, Thomas E. Adrian

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Short-chain fatty acids (SCFAs) reportedly have a trophic effect on the small intestine. However, it is unclear if this is a local or primarily systemic effect. Loss of the ileocolonic junction (ICJ) may result in increased SCFAs and bacteria in the small intestine from colonic reflux. Our aim was to evaluate the effect of bypass of the ICJ on intestinal SCFA content and postresection adaptation. Methods: Thirty dogs were studied: transection control (TC, n = 10), distal resection of 50% intestine (DR, n = 10), and distal resection with bypass of ICJ (DRBP, n = 10). Animals were killed at 4 and 12 weeks. Luminal SCFAs and bacteria and adaptation of the small intestine were evaluated. Results: Caloric intake was significantly less in the two resected groups (67 ± 3 DR and 63 ± 3, DRBP vs 78 ± 5 kcal/kg/dTC, p < .05). Body weight and albumin levels were decreased at 12 weeks but were similar between the resected groups (81% ± 3% and 74% ± 6% initial and 1.9 ± 0.1 and 2.1 ± 0.2 g/dL, DR and DRBP, respectively). Steatorrhea was present for 12 weeks after resection and was greater after DRBP (14.2% ± 3.8% vs 8.6% ± 1.9% at 4 weeks and 13.6% ± 2.5% vs 6.7% ± 0.6% at 12 weeks, p < .05). Bypassed animals had elevated intraluminal SCFA content (3126 ± 1094 vs 1791 ± 538 DR and 1600 ± 446 μg/mL TC, p < .05) and anaerobic bacterial counts (100% vs 50% and 44%, respectively). Tissue inflammation and myeloperoxidase activity were similar. Small intestinal length (174 ± 10 and 180 ± 10 cm) and circumference (5.2 ± 0.4 ± 0.3 cm) increased to a similar extent in both resected groups at 12 weeks. Thickness of mucosa (1939 ± 162 vs 1662 ± 162 μm) and muscle (865 ± 45 vs 978 ± 79 μm) layers were similar after DR and DRBP. Conclusion: (1) Bypass of the ICJ after distal resection results in increased growth of anaerobic bacteria and luminal SCFA and is associated with more marked steatorrhea. (2) Bypass of the ICJ does not influence structural adaptation of the small intestine. (3) These findings do not support a local trophic effect for SCFA.

Original languageEnglish (US)
Pages (from-to)338-343
Number of pages6
JournalJournal of Parenteral and Enteral Nutrition
Volume20
Issue number5
DOIs
StatePublished - Jan 1 1996

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Volatile Fatty Acids
short chain fatty acids
resection
Small Intestine
small intestine
steatorrhea
Steatorrhea
bacteria
fatty acid composition
Bacteria
myeloperoxidase
Anaerobic Bacteria
Bacterial Load
Energy Intake
plate count
Peroxidase
Intestines
mucosa
albumins
Albumins

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Luminal short-chain fatty acids and postresection intestinal adaptation. / Thompson, Jon S; Quigley, Eamonn M.; Palmer, J. M.; West, William W.; Adrian, Thomas E.

In: Journal of Parenteral and Enteral Nutrition, Vol. 20, No. 5, 01.01.1996, p. 338-343.

Research output: Contribution to journalArticle

Thompson, Jon S ; Quigley, Eamonn M. ; Palmer, J. M. ; West, William W. ; Adrian, Thomas E. / Luminal short-chain fatty acids and postresection intestinal adaptation. In: Journal of Parenteral and Enteral Nutrition. 1996 ; Vol. 20, No. 5. pp. 338-343.
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abstract = "Background: Short-chain fatty acids (SCFAs) reportedly have a trophic effect on the small intestine. However, it is unclear if this is a local or primarily systemic effect. Loss of the ileocolonic junction (ICJ) may result in increased SCFAs and bacteria in the small intestine from colonic reflux. Our aim was to evaluate the effect of bypass of the ICJ on intestinal SCFA content and postresection adaptation. Methods: Thirty dogs were studied: transection control (TC, n = 10), distal resection of 50{\%} intestine (DR, n = 10), and distal resection with bypass of ICJ (DRBP, n = 10). Animals were killed at 4 and 12 weeks. Luminal SCFAs and bacteria and adaptation of the small intestine were evaluated. Results: Caloric intake was significantly less in the two resected groups (67 ± 3 DR and 63 ± 3, DRBP vs 78 ± 5 kcal/kg/dTC, p < .05). Body weight and albumin levels were decreased at 12 weeks but were similar between the resected groups (81{\%} ± 3{\%} and 74{\%} ± 6{\%} initial and 1.9 ± 0.1 and 2.1 ± 0.2 g/dL, DR and DRBP, respectively). Steatorrhea was present for 12 weeks after resection and was greater after DRBP (14.2{\%} ± 3.8{\%} vs 8.6{\%} ± 1.9{\%} at 4 weeks and 13.6{\%} ± 2.5{\%} vs 6.7{\%} ± 0.6{\%} at 12 weeks, p < .05). Bypassed animals had elevated intraluminal SCFA content (3126 ± 1094 vs 1791 ± 538 DR and 1600 ± 446 μg/mL TC, p < .05) and anaerobic bacterial counts (100{\%} vs 50{\%} and 44{\%}, respectively). Tissue inflammation and myeloperoxidase activity were similar. Small intestinal length (174 ± 10 and 180 ± 10 cm) and circumference (5.2 ± 0.4 ± 0.3 cm) increased to a similar extent in both resected groups at 12 weeks. Thickness of mucosa (1939 ± 162 vs 1662 ± 162 μm) and muscle (865 ± 45 vs 978 ± 79 μm) layers were similar after DR and DRBP. Conclusion: (1) Bypass of the ICJ after distal resection results in increased growth of anaerobic bacteria and luminal SCFA and is associated with more marked steatorrhea. (2) Bypass of the ICJ does not influence structural adaptation of the small intestine. (3) These findings do not support a local trophic effect for SCFA.",
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N2 - Background: Short-chain fatty acids (SCFAs) reportedly have a trophic effect on the small intestine. However, it is unclear if this is a local or primarily systemic effect. Loss of the ileocolonic junction (ICJ) may result in increased SCFAs and bacteria in the small intestine from colonic reflux. Our aim was to evaluate the effect of bypass of the ICJ on intestinal SCFA content and postresection adaptation. Methods: Thirty dogs were studied: transection control (TC, n = 10), distal resection of 50% intestine (DR, n = 10), and distal resection with bypass of ICJ (DRBP, n = 10). Animals were killed at 4 and 12 weeks. Luminal SCFAs and bacteria and adaptation of the small intestine were evaluated. Results: Caloric intake was significantly less in the two resected groups (67 ± 3 DR and 63 ± 3, DRBP vs 78 ± 5 kcal/kg/dTC, p < .05). Body weight and albumin levels were decreased at 12 weeks but were similar between the resected groups (81% ± 3% and 74% ± 6% initial and 1.9 ± 0.1 and 2.1 ± 0.2 g/dL, DR and DRBP, respectively). Steatorrhea was present for 12 weeks after resection and was greater after DRBP (14.2% ± 3.8% vs 8.6% ± 1.9% at 4 weeks and 13.6% ± 2.5% vs 6.7% ± 0.6% at 12 weeks, p < .05). Bypassed animals had elevated intraluminal SCFA content (3126 ± 1094 vs 1791 ± 538 DR and 1600 ± 446 μg/mL TC, p < .05) and anaerobic bacterial counts (100% vs 50% and 44%, respectively). Tissue inflammation and myeloperoxidase activity were similar. Small intestinal length (174 ± 10 and 180 ± 10 cm) and circumference (5.2 ± 0.4 ± 0.3 cm) increased to a similar extent in both resected groups at 12 weeks. Thickness of mucosa (1939 ± 162 vs 1662 ± 162 μm) and muscle (865 ± 45 vs 978 ± 79 μm) layers were similar after DR and DRBP. Conclusion: (1) Bypass of the ICJ after distal resection results in increased growth of anaerobic bacteria and luminal SCFA and is associated with more marked steatorrhea. (2) Bypass of the ICJ does not influence structural adaptation of the small intestine. (3) These findings do not support a local trophic effect for SCFA.

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