Low temperature surface-enhanced resonance raman scattering (SERRS) from cytochromes

Therese M. Cotton, Vicki L Schlegel, Randall E. Holt, Barbara Swanson, Paul O. de Montellano

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Surface-enhanced resonance Raman scattering (SERRS) studies of cytochrome c (cyt c) and cytochrome P450 (cyt P450) as a function of laser irradiation time have demonstrated that the proteins are extremely sensitive to photodegradation. The results suggest that previous SERRS reports of hemoprotein denaturation on Ag surfaces may reflect photosensitivity rather than an effect of the protein-surface interaction. Photodamage was eliminated by submersion of the electrode into liquid nitrogen. This procedure resulted in stable SERRS spectra, even with prolonged irradiation. The use of a diode array detector also substantially reduces the laser exposure period (< 1 minute) required to observe SERRS spectra of the protein. The application of low temperature SERRS spectroscopy to the study of substrate binding in P450b provided evidence for spin state conversion in the presence of substrate.

Original languageEnglish (US)
Pages (from-to)263-270
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume1055
DOIs
StatePublished - Jul 5 1989

Fingerprint

Raman Spectra
cytochromes
resonance scattering
Cytochromes
Raman scattering
Raman spectra
Proteins
proteins
Temperature
Protein
Irradiation
Substrate
Denaturation
Photosensitivity
irradiation
Laser
biopolymer denaturation
photosensitivity
Photodegradation
Liquid nitrogen

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Low temperature surface-enhanced resonance raman scattering (SERRS) from cytochromes. / Cotton, Therese M.; Schlegel, Vicki L; Holt, Randall E.; Swanson, Barbara; de Montellano, Paul O.

In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 1055, 05.07.1989, p. 263-270.

Research output: Contribution to journalArticle

Cotton, Therese M. ; Schlegel, Vicki L ; Holt, Randall E. ; Swanson, Barbara ; de Montellano, Paul O. / Low temperature surface-enhanced resonance raman scattering (SERRS) from cytochromes. In: Proceedings of SPIE - The International Society for Optical Engineering. 1989 ; Vol. 1055. pp. 263-270.
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