Low-temperature crosslinking of proteins using non-toxic citric acid in neutral aqueous medium

Mechanism and kinetic study

Helan Xu, Li Shen, Lan Xu, Yiqi Yang

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

For the first time, mechanism and kinetics of citric acid crosslinking of protein in neutral aqueous condition under low temperature are reported. Crosslinking was a conventional method to improve properties of protein products to replace petroleum-derived polymers. However, current crosslinking methods were toxic, expensive or required harsh conditions. Non-toxic carboxylic acid could effectively enhance wet performance properties of multiple protein products under mild conditions, which were critical for preserving the activities during immobilization of enzymes. In this study, more than one carboxyl groups in one citric acid molecule were found capable of reacting with protein in aqueous medium at pH 6.8 under 50 and 75°C, verifying occurrence of crosslinking. The pseudo second-order reaction had activation energy as low as 24.956 kJ mol<sup>-1</sup>, indicating its easy occurrence. Mild crosslinking using citric acid might provide alternative to accelerate low-cost and efficient enzyme immobilization as well as industrial utilization of protein-based products.

Original languageEnglish (US)
Pages (from-to)234-240
Number of pages7
JournalIndustrial Crops and Products
Volume74
DOIs
StatePublished - Nov 5 2015

Fingerprint

crosslinking
citric acid
kinetics
immobilized enzymes
protein products
temperature
proteins
water
carboxylic acids
activation energy
petroleum
polymers
methodology

Keywords

  • Aqueous medium
  • Citric acid
  • Kinetics
  • Neutral pH
  • Non-toxic crosslinker
  • Protein

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Low-temperature crosslinking of proteins using non-toxic citric acid in neutral aqueous medium : Mechanism and kinetic study. / Xu, Helan; Shen, Li; Xu, Lan; Yang, Yiqi.

In: Industrial Crops and Products, Vol. 74, 05.11.2015, p. 234-240.

Research output: Contribution to journalArticle

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