Loss of miRNAs during processing and storage of cow's (Bos taurus) milk

Katherine M. Howard, Rio Jati Kusuma, Scott R. Baier, Taylor Friemel, Laura Markham, Jairam Vanamala, Janos Zempleni

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

MicroRNAs (miRs, miRNAs) play central roles in gene regulation. Previously, we reported that miRNAs from pasteurized, store-bought bovine milk have biological activity in humans. Here, we assessed the effects of milk processing, storage, somatic cell content, and handling by consumers on the degradation of miRNAs in milk; we also quantified miRNAs in dairy products. Pasteurization and homogenization caused a 63% loss of miR-200c, whereas a 67% loss observed for miR-29b was statistically significant only in skim milk. Effects of cold storage and somatic cell content were quantitatively minor (<2% loss). Heating in the microwave caused a 40% loss of miR-29b but no loss of miR-200c. The milk fat content had no effect on miRNA stability during storage and microwave heating. The concentrations of miRNAs in dairy products were considerably lower than in store-bought milk. We conclude that processing of milk by dairies and handling by consumers causes a significant loss of miRNAs.

Original languageEnglish (US)
Pages (from-to)588-592
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume63
Issue number2
DOIs
StatePublished - Jan 21 2015

Fingerprint

MicroRNAs
Milk
microwave treatment
microRNA
food processing
somatic cells
cows
milk
dairy products
cattle
Processing
Dairy products
skim milk
pasteurization
homogenization
cold storage
milk fat
Dairy Products
bioactive properties
dairies

Keywords

  • heating
  • miRNAs
  • milk
  • processing
  • storage

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Howard, K. M., Jati Kusuma, R., Baier, S. R., Friemel, T., Markham, L., Vanamala, J., & Zempleni, J. (2015). Loss of miRNAs during processing and storage of cow's (Bos taurus) milk. Journal of Agricultural and Food Chemistry, 63(2), 588-592. https://doi.org/10.1021/jf505526w

Loss of miRNAs during processing and storage of cow's (Bos taurus) milk. / Howard, Katherine M.; Jati Kusuma, Rio; Baier, Scott R.; Friemel, Taylor; Markham, Laura; Vanamala, Jairam; Zempleni, Janos.

In: Journal of Agricultural and Food Chemistry, Vol. 63, No. 2, 21.01.2015, p. 588-592.

Research output: Contribution to journalArticle

Howard, KM, Jati Kusuma, R, Baier, SR, Friemel, T, Markham, L, Vanamala, J & Zempleni, J 2015, 'Loss of miRNAs during processing and storage of cow's (Bos taurus) milk', Journal of Agricultural and Food Chemistry, vol. 63, no. 2, pp. 588-592. https://doi.org/10.1021/jf505526w
Howard KM, Jati Kusuma R, Baier SR, Friemel T, Markham L, Vanamala J et al. Loss of miRNAs during processing and storage of cow's (Bos taurus) milk. Journal of Agricultural and Food Chemistry. 2015 Jan 21;63(2):588-592. https://doi.org/10.1021/jf505526w
Howard, Katherine M. ; Jati Kusuma, Rio ; Baier, Scott R. ; Friemel, Taylor ; Markham, Laura ; Vanamala, Jairam ; Zempleni, Janos. / Loss of miRNAs during processing and storage of cow's (Bos taurus) milk. In: Journal of Agricultural and Food Chemistry. 2015 ; Vol. 63, No. 2. pp. 588-592.
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