Loss of major histocompatibility class II expression in non-immune-privileged site diffuse large B-cell lymphoma is highly coordinated and not due to chromosomal deletions

Lisa M. Rimsza, Robin A. Roberts, Elias Campo, Thomas M. Grogan, Silvia Bea, Itziar Salaverria, Andreas Zettl, Andreas Rosenwald, German Ott, H. Konrad Muller-Hermelink, Jan Delabie, Richard I. Fisher, Joseph M. Unger, Michael LeBlanc, Louis M. Staudt, Elaine S. Jaffe, Randy D. Gascoyne, Wing C. Chan, Dennis D. Weisenburger, Timothy GreinerRita M. Braziel, Thomas P. Miller

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Decreased major histocompatibility class II (MHCII) expression is associated with poor survival in diffuse large B-cell lymphoma (DLBCL). Immune-privileged site DLBCL (IP-DLBCL) patients reportedly have frequent large deletions at the MHCII locus whereas the mechanism of decreased expression in non-IP-DLBCL is unknown. Gene expression profiling data were used for correlation analyses between expression levels of MHCII genes with each other and their transcriptional regulator, CIITA. Comparative genomic hybridization (CGH) assessed chromosomal alterations at MHCII-related loci. Finally, a map was created of expression of genes that are telomeric, within, or centromeric to the MHCII locus. Correlation coefficients among MHCII genes ranged from 0.73 to 0.92, whereas those between adjacent and intervening genes were lower (-0.12 to 0.49). Correlations between MHCII and CIITA expression were higher (0.53 to 0.60) than between CIITA and neighboring genes (-0.05 to 0.22). In 23 MHCII - cases, CGH detected 2 losses and 2 gains at MHCII loci. Expression of genes telomeric, within, and centromeric to MHCII loci were near normal in most MHCII- cases. Large deletions of the MHCII locus are uncommon in non-IP-DLBCL, implicating altered transcription as the operative mechanism for decreased expression.

Original languageEnglish (US)
Pages (from-to)1101-1107
Number of pages7
JournalBlood
Volume107
Issue number3
DOIs
StatePublished - Feb 1 2006

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Histocompatibility
Lymphoma, Large B-Cell, Diffuse
Genes
Cells
MHC Class II Genes
Comparative Genomic Hybridization
Transcription
Gene expression
Gene Expression
Gene Expression Profiling

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Cite this

Loss of major histocompatibility class II expression in non-immune-privileged site diffuse large B-cell lymphoma is highly coordinated and not due to chromosomal deletions. / Rimsza, Lisa M.; Roberts, Robin A.; Campo, Elias; Grogan, Thomas M.; Bea, Silvia; Salaverria, Itziar; Zettl, Andreas; Rosenwald, Andreas; Ott, German; Muller-Hermelink, H. Konrad; Delabie, Jan; Fisher, Richard I.; Unger, Joseph M.; LeBlanc, Michael; Staudt, Louis M.; Jaffe, Elaine S.; Gascoyne, Randy D.; Chan, Wing C.; Weisenburger, Dennis D.; Greiner, Timothy; Braziel, Rita M.; Miller, Thomas P.

In: Blood, Vol. 107, No. 3, 01.02.2006, p. 1101-1107.

Research output: Contribution to journalArticle

Rimsza, LM, Roberts, RA, Campo, E, Grogan, TM, Bea, S, Salaverria, I, Zettl, A, Rosenwald, A, Ott, G, Muller-Hermelink, HK, Delabie, J, Fisher, RI, Unger, JM, LeBlanc, M, Staudt, LM, Jaffe, ES, Gascoyne, RD, Chan, WC, Weisenburger, DD, Greiner, T, Braziel, RM & Miller, TP 2006, 'Loss of major histocompatibility class II expression in non-immune-privileged site diffuse large B-cell lymphoma is highly coordinated and not due to chromosomal deletions', Blood, vol. 107, no. 3, pp. 1101-1107. https://doi.org/10.1182/blood-2005-04-1510
Rimsza, Lisa M. ; Roberts, Robin A. ; Campo, Elias ; Grogan, Thomas M. ; Bea, Silvia ; Salaverria, Itziar ; Zettl, Andreas ; Rosenwald, Andreas ; Ott, German ; Muller-Hermelink, H. Konrad ; Delabie, Jan ; Fisher, Richard I. ; Unger, Joseph M. ; LeBlanc, Michael ; Staudt, Louis M. ; Jaffe, Elaine S. ; Gascoyne, Randy D. ; Chan, Wing C. ; Weisenburger, Dennis D. ; Greiner, Timothy ; Braziel, Rita M. ; Miller, Thomas P. / Loss of major histocompatibility class II expression in non-immune-privileged site diffuse large B-cell lymphoma is highly coordinated and not due to chromosomal deletions. In: Blood. 2006 ; Vol. 107, No. 3. pp. 1101-1107.
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abstract = "Decreased major histocompatibility class II (MHCII) expression is associated with poor survival in diffuse large B-cell lymphoma (DLBCL). Immune-privileged site DLBCL (IP-DLBCL) patients reportedly have frequent large deletions at the MHCII locus whereas the mechanism of decreased expression in non-IP-DLBCL is unknown. Gene expression profiling data were used for correlation analyses between expression levels of MHCII genes with each other and their transcriptional regulator, CIITA. Comparative genomic hybridization (CGH) assessed chromosomal alterations at MHCII-related loci. Finally, a map was created of expression of genes that are telomeric, within, or centromeric to the MHCII locus. Correlation coefficients among MHCII genes ranged from 0.73 to 0.92, whereas those between adjacent and intervening genes were lower (-0.12 to 0.49). Correlations between MHCII and CIITA expression were higher (0.53 to 0.60) than between CIITA and neighboring genes (-0.05 to 0.22). In 23 MHCII - cases, CGH detected 2 losses and 2 gains at MHCII loci. Expression of genes telomeric, within, and centromeric to MHCII loci were near normal in most MHCII- cases. Large deletions of the MHCII locus are uncommon in non-IP-DLBCL, implicating altered transcription as the operative mechanism for decreased expression.",
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T1 - Loss of major histocompatibility class II expression in non-immune-privileged site diffuse large B-cell lymphoma is highly coordinated and not due to chromosomal deletions

AU - Rimsza, Lisa M.

AU - Roberts, Robin A.

AU - Campo, Elias

AU - Grogan, Thomas M.

AU - Bea, Silvia

AU - Salaverria, Itziar

AU - Zettl, Andreas

AU - Rosenwald, Andreas

AU - Ott, German

AU - Muller-Hermelink, H. Konrad

AU - Delabie, Jan

AU - Fisher, Richard I.

AU - Unger, Joseph M.

AU - LeBlanc, Michael

AU - Staudt, Louis M.

AU - Jaffe, Elaine S.

AU - Gascoyne, Randy D.

AU - Chan, Wing C.

AU - Weisenburger, Dennis D.

AU - Greiner, Timothy

AU - Braziel, Rita M.

AU - Miller, Thomas P.

PY - 2006/2/1

Y1 - 2006/2/1

N2 - Decreased major histocompatibility class II (MHCII) expression is associated with poor survival in diffuse large B-cell lymphoma (DLBCL). Immune-privileged site DLBCL (IP-DLBCL) patients reportedly have frequent large deletions at the MHCII locus whereas the mechanism of decreased expression in non-IP-DLBCL is unknown. Gene expression profiling data were used for correlation analyses between expression levels of MHCII genes with each other and their transcriptional regulator, CIITA. Comparative genomic hybridization (CGH) assessed chromosomal alterations at MHCII-related loci. Finally, a map was created of expression of genes that are telomeric, within, or centromeric to the MHCII locus. Correlation coefficients among MHCII genes ranged from 0.73 to 0.92, whereas those between adjacent and intervening genes were lower (-0.12 to 0.49). Correlations between MHCII and CIITA expression were higher (0.53 to 0.60) than between CIITA and neighboring genes (-0.05 to 0.22). In 23 MHCII - cases, CGH detected 2 losses and 2 gains at MHCII loci. Expression of genes telomeric, within, and centromeric to MHCII loci were near normal in most MHCII- cases. Large deletions of the MHCII locus are uncommon in non-IP-DLBCL, implicating altered transcription as the operative mechanism for decreased expression.

AB - Decreased major histocompatibility class II (MHCII) expression is associated with poor survival in diffuse large B-cell lymphoma (DLBCL). Immune-privileged site DLBCL (IP-DLBCL) patients reportedly have frequent large deletions at the MHCII locus whereas the mechanism of decreased expression in non-IP-DLBCL is unknown. Gene expression profiling data were used for correlation analyses between expression levels of MHCII genes with each other and their transcriptional regulator, CIITA. Comparative genomic hybridization (CGH) assessed chromosomal alterations at MHCII-related loci. Finally, a map was created of expression of genes that are telomeric, within, or centromeric to the MHCII locus. Correlation coefficients among MHCII genes ranged from 0.73 to 0.92, whereas those between adjacent and intervening genes were lower (-0.12 to 0.49). Correlations between MHCII and CIITA expression were higher (0.53 to 0.60) than between CIITA and neighboring genes (-0.05 to 0.22). In 23 MHCII - cases, CGH detected 2 losses and 2 gains at MHCII loci. Expression of genes telomeric, within, and centromeric to MHCII loci were near normal in most MHCII- cases. Large deletions of the MHCII locus are uncommon in non-IP-DLBCL, implicating altered transcription as the operative mechanism for decreased expression.

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