Loss of infectivity by progeny virus from alpha interferon-treated human immunodeficiency virus type 1-infected T cells is associated with defective assembly of envelope gp120

B. D. Hansen, P. L. Nara, R. K. Maheshwari, G. S. Sidhu, J. G. Bernbaum, D. Hoekzema, M. S. Meltzer, Howard Eliot Gendelman

Research output: Contribution to journalComment/debate

65 Citations (Scopus)

Abstract

Levels of human immunodeficiency virus (HIV) DNA, RNA, or p24 antigen and reverse transcriptase activity in T-cell cultures treated with 500 IU of recombinant alpha interferon (rIFNα) per ml were comparable to those in control cultures. Radioimmunoprecipitation analysis of proteins in lysates of IFN-treated T cells documented a marked accumulation of HIV proteins. Localization of gp120 by immunofluorescence showed a diffuse pattern in IFN- treated cells quite distinct from the ring pattern in untreated control cells. That large quantities of gp120 in aberrant cell compartments might affect HIV morphogenesis was confirmed in infectivity studies: virions from IFN-treated cells were 100- to 1,000-fold less infectious than an equal number of virions from control cells. Direct examination of IFN-treated and control HIV-infected cells by transmission electron microscopy showed little difference in the number or distribution of viral particles. However, quantitation of gp120 by immunogold particle analysis revealed a marked depletion of envelope glycoprotein in virions released from IFN-treated cells. This defect in gp120 assembly onto mature viral particles provides a molecular basis for this loss of infectivity.

Original languageEnglish (US)
Pages (from-to)7543-7548
Number of pages6
JournalJournal of virology
Volume66
Issue number12
StatePublished - Jan 1 1992

Fingerprint

interferon-alpha
Human immunodeficiency virus 1
Interferon-alpha
HIV-1
pathogenicity
T-lymphocytes
virion
Viruses
Virion
T-Lymphocytes
Human immunodeficiency virus
viruses
cells
HIV
Radioimmunoprecipitation Assay
Human Immunodeficiency Virus Proteins
RNA-directed DNA polymerase
RNA-Directed DNA Polymerase
Transmission Electron Microscopy
Morphogenesis

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Loss of infectivity by progeny virus from alpha interferon-treated human immunodeficiency virus type 1-infected T cells is associated with defective assembly of envelope gp120. / Hansen, B. D.; Nara, P. L.; Maheshwari, R. K.; Sidhu, G. S.; Bernbaum, J. G.; Hoekzema, D.; Meltzer, M. S.; Gendelman, Howard Eliot.

In: Journal of virology, Vol. 66, No. 12, 01.01.1992, p. 7543-7548.

Research output: Contribution to journalComment/debate

Hansen, B. D. ; Nara, P. L. ; Maheshwari, R. K. ; Sidhu, G. S. ; Bernbaum, J. G. ; Hoekzema, D. ; Meltzer, M. S. ; Gendelman, Howard Eliot. / Loss of infectivity by progeny virus from alpha interferon-treated human immunodeficiency virus type 1-infected T cells is associated with defective assembly of envelope gp120. In: Journal of virology. 1992 ; Vol. 66, No. 12. pp. 7543-7548.
@article{a92d62161f6c42358054b7d5f541d41a,
title = "Loss of infectivity by progeny virus from alpha interferon-treated human immunodeficiency virus type 1-infected T cells is associated with defective assembly of envelope gp120",
abstract = "Levels of human immunodeficiency virus (HIV) DNA, RNA, or p24 antigen and reverse transcriptase activity in T-cell cultures treated with 500 IU of recombinant alpha interferon (rIFNα) per ml were comparable to those in control cultures. Radioimmunoprecipitation analysis of proteins in lysates of IFN-treated T cells documented a marked accumulation of HIV proteins. Localization of gp120 by immunofluorescence showed a diffuse pattern in IFN- treated cells quite distinct from the ring pattern in untreated control cells. That large quantities of gp120 in aberrant cell compartments might affect HIV morphogenesis was confirmed in infectivity studies: virions from IFN-treated cells were 100- to 1,000-fold less infectious than an equal number of virions from control cells. Direct examination of IFN-treated and control HIV-infected cells by transmission electron microscopy showed little difference in the number or distribution of viral particles. However, quantitation of gp120 by immunogold particle analysis revealed a marked depletion of envelope glycoprotein in virions released from IFN-treated cells. This defect in gp120 assembly onto mature viral particles provides a molecular basis for this loss of infectivity.",
author = "Hansen, {B. D.} and Nara, {P. L.} and Maheshwari, {R. K.} and Sidhu, {G. S.} and Bernbaum, {J. G.} and D. Hoekzema and Meltzer, {M. S.} and Gendelman, {Howard Eliot}",
year = "1992",
month = "1",
day = "1",
language = "English (US)",
volume = "66",
pages = "7543--7548",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "12",

}

TY - JOUR

T1 - Loss of infectivity by progeny virus from alpha interferon-treated human immunodeficiency virus type 1-infected T cells is associated with defective assembly of envelope gp120

AU - Hansen, B. D.

AU - Nara, P. L.

AU - Maheshwari, R. K.

AU - Sidhu, G. S.

AU - Bernbaum, J. G.

AU - Hoekzema, D.

AU - Meltzer, M. S.

AU - Gendelman, Howard Eliot

PY - 1992/1/1

Y1 - 1992/1/1

N2 - Levels of human immunodeficiency virus (HIV) DNA, RNA, or p24 antigen and reverse transcriptase activity in T-cell cultures treated with 500 IU of recombinant alpha interferon (rIFNα) per ml were comparable to those in control cultures. Radioimmunoprecipitation analysis of proteins in lysates of IFN-treated T cells documented a marked accumulation of HIV proteins. Localization of gp120 by immunofluorescence showed a diffuse pattern in IFN- treated cells quite distinct from the ring pattern in untreated control cells. That large quantities of gp120 in aberrant cell compartments might affect HIV morphogenesis was confirmed in infectivity studies: virions from IFN-treated cells were 100- to 1,000-fold less infectious than an equal number of virions from control cells. Direct examination of IFN-treated and control HIV-infected cells by transmission electron microscopy showed little difference in the number or distribution of viral particles. However, quantitation of gp120 by immunogold particle analysis revealed a marked depletion of envelope glycoprotein in virions released from IFN-treated cells. This defect in gp120 assembly onto mature viral particles provides a molecular basis for this loss of infectivity.

AB - Levels of human immunodeficiency virus (HIV) DNA, RNA, or p24 antigen and reverse transcriptase activity in T-cell cultures treated with 500 IU of recombinant alpha interferon (rIFNα) per ml were comparable to those in control cultures. Radioimmunoprecipitation analysis of proteins in lysates of IFN-treated T cells documented a marked accumulation of HIV proteins. Localization of gp120 by immunofluorescence showed a diffuse pattern in IFN- treated cells quite distinct from the ring pattern in untreated control cells. That large quantities of gp120 in aberrant cell compartments might affect HIV morphogenesis was confirmed in infectivity studies: virions from IFN-treated cells were 100- to 1,000-fold less infectious than an equal number of virions from control cells. Direct examination of IFN-treated and control HIV-infected cells by transmission electron microscopy showed little difference in the number or distribution of viral particles. However, quantitation of gp120 by immunogold particle analysis revealed a marked depletion of envelope glycoprotein in virions released from IFN-treated cells. This defect in gp120 assembly onto mature viral particles provides a molecular basis for this loss of infectivity.

UR - http://www.scopus.com/inward/record.url?scp=0026464669&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026464669&partnerID=8YFLogxK

M3 - Comment/debate

VL - 66

SP - 7543

EP - 7548

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 12

ER -