Loss of E-cadherin and epithelial to mesenchymal transition is not required for cell motility in tissues or for metastasis

Xiang Liu, Huocong Huang, Neeley Remmers, Michael A Hollingsworth

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Loss of E-cadherin has been long considered to be a major hallmark of epithelial- mesenchymal transition (EMT) and has been reported in various cancers. P120 catenin regulates E-cadherin stability on the cell surface and also plays a role in intracellular signaling by modulating nuclear transcription. We recently characterized the nature of interactions between p120 catenin and Mucin 1 (MUC1) in pancreatic cancer. Expression of different p120 catenin isoforms with and without MUC1 induced distinct morphologies, cell adhesion, and dynamic properties of motility along with different metastatic properties in vivo. Re-expression of p120 catenin isoform 3A in the context of MUC1 expression in a p120 catenin-deficient cell line stabilized expression of E-cadherin. However, orthotopic implantation of tumors using this stable cell line produced large metastatic lesions to the liver, which exceeded the volume of the primary tumor, suggesting down regulation of E-cadherin is not required for tumor metastasis. Here we extend those studies by showing that ectopic expression of E-cadherin does not block in vitro invasion of the pancreatic cancer cells, and instead accelerated the rate of tumor invasion. Furthermore, results from 23 cases of human pancreatic primary tumor specimens revealed that most tumors exhibiting metastatic activity retained epithelial morphology and E-cadherin gene expression. Our results indicate that loss of E-cadherin and EMT are not required for metastasis and that an epithelial morphology can be maintained during the process of tumor cell movement.

Original languageEnglish (US)
Article numbere969112
JournalTissue Barriers
Volume2
Issue number4
DOIs
StatePublished - Nov 14 2014

Fingerprint

Epithelial-Mesenchymal Transition
Cadherins
Cell Movement
Tumors
Tissue
Neoplasm Metastasis
Mucin-1
Cells
Neoplasms
Pancreatic Neoplasms
Protein Isoforms
Cell Line
Cell adhesion
Transcription
Tumor Burden
Gene expression
Cell Adhesion
Liver
Down-Regulation
delta catenin

Keywords

  • Cell motility
  • E-cadherin
  • Epithelial to Mesenchymal Transition
  • MUC1
  • P120 catenin
  • Pancreatic cancer Metastasis

ASJC Scopus subject areas

  • Biochemistry
  • Histology
  • Cell Biology

Cite this

Loss of E-cadherin and epithelial to mesenchymal transition is not required for cell motility in tissues or for metastasis. / Liu, Xiang; Huang, Huocong; Remmers, Neeley; Hollingsworth, Michael A.

In: Tissue Barriers, Vol. 2, No. 4, e969112, 14.11.2014.

Research output: Contribution to journalArticle

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