Longitudinal analysis of mammogenesis using a novel tetracycline-inducible mouse model and in vivo imaging

Bradley A. Creamer, Aleata A. Triplett, Kay Uwe Wagner

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We generated a novel mouse model, which expresses the tetracycline-inducible transactivator under the regulation of the endogenous whey acidic protein gene. Using a tet-responsive luciferase reporter transgene, we demonstrated that the Wap-rtTA knockin allele allows a tightly controlled temporal and spatial expression of transgenes in the mammary gland in a ligand-inducible manner. The longitudinal analysis of individual females throughout their reproductive cycles using in vivo bioluminescence imaging confirmed that the expression of the Wap-rtTA knockin allele is highly upregulated during lactation. However, the extent of the transcriptional activation of the targeted Wap locus is dependent on the suckling stimulus and milk retrieval. In addition, we used WAP-rtTA/TetO-H2B-GFP double-transgenic females to monitor the presence of GFP-labeled parity-induced mammary epithelial cells (PI-MECs) during the postlactational involution period. The study shows that, unlike their progeny in mammary epithelial transplants as reported previously, PI-MECs themselves may not belong to the long-term label-retaining epithelial subtype.

Original languageEnglish (US)
Pages (from-to)234-245
Number of pages12
JournalGenesis
Volume47
Issue number4
DOIs
StatePublished - Jun 3 2009

Fingerprint

Tetracycline
Breast
Parity
Transgenes
Epithelial Cells
Alleles
Trans-Activators
Human Mammary Glands
Luciferases
Lactation
Transcriptional Activation
Milk
Ligands
Transplants
Genes

Keywords

  • Differentiation
  • Gene targeting
  • In vivo imaging
  • Lactation
  • Mammary gland development
  • Tetracycline-inducible transactivator
  • Whey acidic protein

ASJC Scopus subject areas

  • Genetics
  • Endocrinology
  • Cell Biology

Cite this

Longitudinal analysis of mammogenesis using a novel tetracycline-inducible mouse model and in vivo imaging. / Creamer, Bradley A.; Triplett, Aleata A.; Wagner, Kay Uwe.

In: Genesis, Vol. 47, No. 4, 03.06.2009, p. 234-245.

Research output: Contribution to journalArticle

Creamer, Bradley A. ; Triplett, Aleata A. ; Wagner, Kay Uwe. / Longitudinal analysis of mammogenesis using a novel tetracycline-inducible mouse model and in vivo imaging. In: Genesis. 2009 ; Vol. 47, No. 4. pp. 234-245.
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