Long-term activity of biohybrid coatings of atrazine-degrading bacteria Pseudomonas sp. ADP

A. Pannier, T. Lehrer, M. Vogel, U. Soltmann, H. Böttcher, S. Tarre, M. Green, J. Raff, K. Pollmann

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The atrazine-degrading bacterial strain Pseudomonas sp. ADP was immobilized by the sol-gel process within thin silica layers coated onto water-retaining carrier materials (expanded clay pellets and scoria). The performance of the obtained biohybrid material has been investigated concerning long-term activity under non-growth conditions. Experiments were run in phosphate buffer containing atrazine (20 mg l-1) as the sole source of carbon and nitrogen. Even after one year of consecutive batch tests, P. ADP immobilized onto expanded clay pellets showed a high atrazine degradation activity. In the course of long-term batch experiments, the average amount of removed atrazine was about 94% during each assay cycle. Staining with CTC revealed that in spite of cultivation under non-growth conditions over a period of one year, immobilized cells were still vital and showed respiratory activity.

Original languageEnglish (US)
Pages (from-to)19970-19979
Number of pages10
JournalRSC Advances
Volume4
Issue number38
DOIs
StatePublished - 2014

Fingerprint

Atrazine
Administrative data processing
Herbicides
Adenosine Diphosphate
Bacteria
Coatings
Clay
Silicon Dioxide
Sol-gel process
Assays
Buffers
Phosphates
Nitrogen
Carbon
Experiments
Silica
Cells
Degradation
Water
clay

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Pannier, A., Lehrer, T., Vogel, M., Soltmann, U., Böttcher, H., Tarre, S., ... Pollmann, K. (2014). Long-term activity of biohybrid coatings of atrazine-degrading bacteria Pseudomonas sp. ADP. RSC Advances, 4(38), 19970-19979. https://doi.org/10.1039/c4ra02928c

Long-term activity of biohybrid coatings of atrazine-degrading bacteria Pseudomonas sp. ADP. / Pannier, A.; Lehrer, T.; Vogel, M.; Soltmann, U.; Böttcher, H.; Tarre, S.; Green, M.; Raff, J.; Pollmann, K.

In: RSC Advances, Vol. 4, No. 38, 2014, p. 19970-19979.

Research output: Contribution to journalArticle

Pannier, A, Lehrer, T, Vogel, M, Soltmann, U, Böttcher, H, Tarre, S, Green, M, Raff, J & Pollmann, K 2014, 'Long-term activity of biohybrid coatings of atrazine-degrading bacteria Pseudomonas sp. ADP', RSC Advances, vol. 4, no. 38, pp. 19970-19979. https://doi.org/10.1039/c4ra02928c
Pannier, A. ; Lehrer, T. ; Vogel, M. ; Soltmann, U. ; Böttcher, H. ; Tarre, S. ; Green, M. ; Raff, J. ; Pollmann, K. / Long-term activity of biohybrid coatings of atrazine-degrading bacteria Pseudomonas sp. ADP. In: RSC Advances. 2014 ; Vol. 4, No. 38. pp. 19970-19979.
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