Location of T-cell epitopes in nonstructural proteins 9 and 10 of type-II porcine reproductive and respiratory syndrome virus

Rajeshwari Parida, In Soo Choi, Daniel A. Peterson, Asit K Pattnaik, William Laegreid, Federico A. Zuckermann, Fernando A. Osorio

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is a significant swine pathogen which exhibits considerable sequence diversity. In an attempt to identify highly conserved T-cell epitopes contained in proteins of this virus, we examined heptadecamer peptides spanning the sequence of the PRRSV nonstructural proteins (NSPs) 9 and 10, both of which are highly conserved, for their ability to elicit a recall proliferative and interferon-gamma response in peripheral blood mononuclear cells obtained from pigs immunized against the type-II PRRSV strain FL-12. These studies led to the identification of four peptides, two from each NSP9 and NSP10 that appear to contain T-cell epitopes. Comparison of the amino acid sequence of these four peptide sequences to the analogous sequences from a diverse sample of type-II PRRSV strains indicated that these sequences are highly conserved and thus contain highly conserved T-cell epitopes. The identified epitopes may be important in the formulation of immunogens to provide broad cross-protection against diverse PRRSV strains.

Original languageEnglish (US)
Pages (from-to)13-21
Number of pages9
JournalVirus Research
Volume169
Issue number1
DOIs
StatePublished - Oct 1 2012

Fingerprint

Porcine respiratory and reproductive syndrome virus
T-Lymphocyte Epitopes
Proteins
Peptides
Swine
Cross Protection
Conserved Sequence
Interferon-gamma
Epitopes
Amino Acid Sequence
Blood Cells
Viruses

Keywords

  • Nonstructural proteins
  • PRRSV vaccines
  • Porcine reproductive and respiratory syndrome virus
  • T-cell epitopes

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

Cite this

Location of T-cell epitopes in nonstructural proteins 9 and 10 of type-II porcine reproductive and respiratory syndrome virus. / Parida, Rajeshwari; Choi, In Soo; Peterson, Daniel A.; Pattnaik, Asit K; Laegreid, William; Zuckermann, Federico A.; Osorio, Fernando A.

In: Virus Research, Vol. 169, No. 1, 01.10.2012, p. 13-21.

Research output: Contribution to journalArticle

Parida, Rajeshwari ; Choi, In Soo ; Peterson, Daniel A. ; Pattnaik, Asit K ; Laegreid, William ; Zuckermann, Federico A. ; Osorio, Fernando A. / Location of T-cell epitopes in nonstructural proteins 9 and 10 of type-II porcine reproductive and respiratory syndrome virus. In: Virus Research. 2012 ; Vol. 169, No. 1. pp. 13-21.
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AU - Laegreid, William

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