Localization of the inositol 1,4,5-trisphosphate receptor in synaptic terminals in the vertebrate retina

Y. W. Peng, A. H. Sharp, S. H. Snyder, K. W. Yau

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Inositol 1,4,5-trisphosphate (InsP3) mobilizes internal Ca2+ in cells by binding to a receptor protein, which has recently been purified and molecularly cloned. To clarify those neuronal functions that are regulated by InsP3, we have localized this (InsP3) receptor protein immunocytochemically in the retina, a neural tissue of well-defined structure and function. Positive staining in neurons is confined almost exclusively to the synaptic layers. Using dissociated retinal neurons, we have further localized the receptor to presynaptic terminals of photoreceptors and bipolar cells, as well as the synaptic processes of amacrine cells. The specific association of InsP3 receptors with synaptic terminals suggests a role for InsP3 in synaptic modulation, especially with respect to transmitter release.

Original languageEnglish (US)
Pages (from-to)525-531
Number of pages7
JournalNeuron
Volume6
Issue number4
DOIs
StatePublished - Apr 1991

Fingerprint

Inositol 1,4,5-Trisphosphate Receptors
Presynaptic Terminals
Vertebrates
Retina
Retinal Neurons
Amacrine Cells
Photoreceptor Cells
Inositol 1,4,5-Trisphosphate
Proteins
Staining and Labeling
Neurons

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Localization of the inositol 1,4,5-trisphosphate receptor in synaptic terminals in the vertebrate retina. / Peng, Y. W.; Sharp, A. H.; Snyder, S. H.; Yau, K. W.

In: Neuron, Vol. 6, No. 4, 04.1991, p. 525-531.

Research output: Contribution to journalArticle

Peng, Y. W. ; Sharp, A. H. ; Snyder, S. H. ; Yau, K. W. / Localization of the inositol 1,4,5-trisphosphate receptor in synaptic terminals in the vertebrate retina. In: Neuron. 1991 ; Vol. 6, No. 4. pp. 525-531.
@article{a325dd20a10a4cfe8a8dd982c3861f81,
title = "Localization of the inositol 1,4,5-trisphosphate receptor in synaptic terminals in the vertebrate retina",
abstract = "Inositol 1,4,5-trisphosphate (InsP3) mobilizes internal Ca2+ in cells by binding to a receptor protein, which has recently been purified and molecularly cloned. To clarify those neuronal functions that are regulated by InsP3, we have localized this (InsP3) receptor protein immunocytochemically in the retina, a neural tissue of well-defined structure and function. Positive staining in neurons is confined almost exclusively to the synaptic layers. Using dissociated retinal neurons, we have further localized the receptor to presynaptic terminals of photoreceptors and bipolar cells, as well as the synaptic processes of amacrine cells. The specific association of InsP3 receptors with synaptic terminals suggests a role for InsP3 in synaptic modulation, especially with respect to transmitter release.",
author = "Peng, {Y. W.} and Sharp, {A. H.} and Snyder, {S. H.} and Yau, {K. W.}",
year = "1991",
month = "4",
doi = "10.1016/0896-6273(91)90055-5",
language = "English (US)",
volume = "6",
pages = "525--531",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "4",

}

TY - JOUR

T1 - Localization of the inositol 1,4,5-trisphosphate receptor in synaptic terminals in the vertebrate retina

AU - Peng, Y. W.

AU - Sharp, A. H.

AU - Snyder, S. H.

AU - Yau, K. W.

PY - 1991/4

Y1 - 1991/4

N2 - Inositol 1,4,5-trisphosphate (InsP3) mobilizes internal Ca2+ in cells by binding to a receptor protein, which has recently been purified and molecularly cloned. To clarify those neuronal functions that are regulated by InsP3, we have localized this (InsP3) receptor protein immunocytochemically in the retina, a neural tissue of well-defined structure and function. Positive staining in neurons is confined almost exclusively to the synaptic layers. Using dissociated retinal neurons, we have further localized the receptor to presynaptic terminals of photoreceptors and bipolar cells, as well as the synaptic processes of amacrine cells. The specific association of InsP3 receptors with synaptic terminals suggests a role for InsP3 in synaptic modulation, especially with respect to transmitter release.

AB - Inositol 1,4,5-trisphosphate (InsP3) mobilizes internal Ca2+ in cells by binding to a receptor protein, which has recently been purified and molecularly cloned. To clarify those neuronal functions that are regulated by InsP3, we have localized this (InsP3) receptor protein immunocytochemically in the retina, a neural tissue of well-defined structure and function. Positive staining in neurons is confined almost exclusively to the synaptic layers. Using dissociated retinal neurons, we have further localized the receptor to presynaptic terminals of photoreceptors and bipolar cells, as well as the synaptic processes of amacrine cells. The specific association of InsP3 receptors with synaptic terminals suggests a role for InsP3 in synaptic modulation, especially with respect to transmitter release.

UR - http://www.scopus.com/inward/record.url?scp=0025805226&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025805226&partnerID=8YFLogxK

U2 - 10.1016/0896-6273(91)90055-5

DO - 10.1016/0896-6273(91)90055-5

M3 - Article

C2 - 1849721

AN - SCOPUS:0025805226

VL - 6

SP - 525

EP - 531

JO - Neuron

JF - Neuron

SN - 0896-6273

IS - 4

ER -