Localization of the human fibronectin (FN) gene on chromosome 8 by a specific enzyme immunoassay

Stephen Israel Rennard, Robert L. Church, David H. Rohrbach, Dolores E. Shupp, Shigeto Abe, A. Tyl Hewitt, J. Clifford Murray, George R. Martin

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The fibronectin produced by clonal murine-human hybrid cell lines containing various complements of human chromosomes was measured. Human and murine fibronectins were assayed by specific immunoassay, and the production of human fibronectin was correlated with karyology and isozyme markers for specific human chromosomes. The data show a 100% concordance between the expression of human fibronectin and glutathione reductase, a marker for human chromosome 8, indicating that chromosome 8 codes for the fibronectin polypeptide.

Original languageEnglish (US)
Pages (from-to)551-566
Number of pages16
JournalBiochemical Genetics
Volume19
Issue number5-6
DOIs
StatePublished - Jun 1 1981

Fingerprint

Chromosomes, Human, Pair 8
fibronectins
immunoassay
enzyme immunoassays
Chromosomes
Immunoenzyme Techniques
Fibronectins
chromosome
Genes
enzyme
chromosomes
Human Chromosomes
gene
Enzymes
genes
karyology
Glutathione Reductase
Hybrid Cells
Immunoassay
Isoenzymes

Keywords

  • ELISA
  • fibronecting
  • gene mapping
  • murine-human hybrids

ASJC Scopus subject areas

  • Biochemistry
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

Cite this

Rennard, S. I., Church, R. L., Rohrbach, D. H., Shupp, D. E., Abe, S., Hewitt, A. T., ... Martin, G. R. (1981). Localization of the human fibronectin (FN) gene on chromosome 8 by a specific enzyme immunoassay. Biochemical Genetics, 19(5-6), 551-566. https://doi.org/10.1007/BF00484626

Localization of the human fibronectin (FN) gene on chromosome 8 by a specific enzyme immunoassay. / Rennard, Stephen Israel; Church, Robert L.; Rohrbach, David H.; Shupp, Dolores E.; Abe, Shigeto; Hewitt, A. Tyl; Murray, J. Clifford; Martin, George R.

In: Biochemical Genetics, Vol. 19, No. 5-6, 01.06.1981, p. 551-566.

Research output: Contribution to journalArticle

Rennard, SI, Church, RL, Rohrbach, DH, Shupp, DE, Abe, S, Hewitt, AT, Murray, JC & Martin, GR 1981, 'Localization of the human fibronectin (FN) gene on chromosome 8 by a specific enzyme immunoassay', Biochemical Genetics, vol. 19, no. 5-6, pp. 551-566. https://doi.org/10.1007/BF00484626
Rennard SI, Church RL, Rohrbach DH, Shupp DE, Abe S, Hewitt AT et al. Localization of the human fibronectin (FN) gene on chromosome 8 by a specific enzyme immunoassay. Biochemical Genetics. 1981 Jun 1;19(5-6):551-566. https://doi.org/10.1007/BF00484626
Rennard, Stephen Israel ; Church, Robert L. ; Rohrbach, David H. ; Shupp, Dolores E. ; Abe, Shigeto ; Hewitt, A. Tyl ; Murray, J. Clifford ; Martin, George R. / Localization of the human fibronectin (FN) gene on chromosome 8 by a specific enzyme immunoassay. In: Biochemical Genetics. 1981 ; Vol. 19, No. 5-6. pp. 551-566.
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