Localization of myeloperoxidase to the long arm of human chromosome 17

Relationship to the 15;17 translocation of acute promyelocytic leukemia

P. Van Tuinen, Keith R Johnson, S. A. Ledbetter, R. L. Nussbaum, G. Rovera, D. H. Ledbetter

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Myeloperoxidase (MPO) is an enzyme whose synthesis is restricted to the promyelocytic stage of myeloid differentiation. We have recently described the cloning and sequencing of a cDNA for MPO. Using a regional mapping panel of somatic cell hybrids containing various deleted or translocated segments of chromosome 17, we have assigned MPO to a region between 17q21 and 17q23. In situ hybridization refined this localization in that grains on chromosome 17 were significantly clustered at bands q22-23 and no hybridization was detected at q21. In light of this chromosome assignment, the relationship of MPO to the 17q translocation breakpoint characteristic of acute promyelocytic leukemia (APL) was considered. Because this breakpoint has been variously assigned to different bands on 17q from 17q11.2 to 17q22, the cytogenetic and molecular distance between this breakpoint and MPO cannot be accurately determined. MPO and other probes mapped to this region of 17 will be important in searching for altered Southern blot patterns after conventional or pulsed-field gel analysis of DNA from APL patients.

Original languageEnglish (US)
Pages (from-to)319-322
Number of pages4
JournalOncogene
Volume1
Issue number3
StatePublished - Jan 1 1987

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Acute Promyelocytic Leukemia
Chromosomes, Human, Pair 17
Human Chromosomes
Peroxidase
Hybrid Cells
Southern Blotting
Cytogenetics
In Situ Hybridization
Organism Cloning
Complementary DNA
Chromosomes
Gels
DNA
Enzymes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Localization of myeloperoxidase to the long arm of human chromosome 17 : Relationship to the 15;17 translocation of acute promyelocytic leukemia. / Van Tuinen, P.; Johnson, Keith R; Ledbetter, S. A.; Nussbaum, R. L.; Rovera, G.; Ledbetter, D. H.

In: Oncogene, Vol. 1, No. 3, 01.01.1987, p. 319-322.

Research output: Contribution to journalArticle

Van Tuinen, P. ; Johnson, Keith R ; Ledbetter, S. A. ; Nussbaum, R. L. ; Rovera, G. ; Ledbetter, D. H. / Localization of myeloperoxidase to the long arm of human chromosome 17 : Relationship to the 15;17 translocation of acute promyelocytic leukemia. In: Oncogene. 1987 ; Vol. 1, No. 3. pp. 319-322.
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