Localization of low-melting regions in phage T7 DNA

V. I. Lyamichev, I. G. Panyutin, D. I. Cherny, Yu L. Lyubchenko

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Abstract

Specific fragmentation of T7 DNA at glyoxal-fixed denatured regions by the S1 endonuclease followed by restriction analysis made it possible to localize four low-melting regions in phage T7 DNA. These regions have the following coordinates:0.5-1.2;14.8±0.3;46.3±0.5; 98.4±0.3 (in T7 DNA length units). The location of the low-melting regions was refined by means of electron-microscopic denaturation mapping and gel electrophoresis of partially denatured DNA. The obtained localization of the low-melting regions is consistent with the available data on the sequence of T7 DNA. The map of low-melting regions was compared with the genetic map of T7 DNA.

Original languageEnglish (US)
Pages (from-to)2165-2176
Number of pages12
JournalNucleic acids research
Volume11
Issue number7
DOIs
Publication statusPublished - Apr 11 1983

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ASJC Scopus subject areas

  • Genetics

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