Localization of galectin-3 in normal and diseased pancreatic tissue

Courtney Schaffert, Parviz M. Pour, William G. Chaney

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Conclusion. Galectin-3 is expressed in both human and hamster pancreatic tumors and tumor cell lines and this expression is increased over normal. Background. Galectin-3 is overexpressed in many gastrointestinal tumors. This study examined the expression of galectin-3 in human and hamster pancreatic tumors to determine if galectin-3 could be used as a marker for pancreatic cancer. Methods. Membranes were prepared from human and hamster pancreatic tumor cell lines. Galectin-3 was visualized by immunoblot analysis of separated membrane proteins using the monoclonal antibody (MAb) M3/38. Paraffin-embedded sections from normal, pancreatitis, and cancerous human pancreatic tissue and normal, N-nitrosobis(2-oxopropyl)amine (BOP)-treated hyperplastic, and cancerous hamster pancreatic tissues were processed immunohistochemically for galectin-3 using the MAb M3/38. Results. Galectin- 3 was heavily expressed in cytoplasmic and nuclear regions of 50% of normal human pancreatic tissue. Expression of galectin-3 in ductal cells in chronic pancreatitis and cancerous pancreatic tissue was increased over normal and was more uniform (>95% cells/duct stained). Normal hamster pancreatic ducts showed weak or no expression of galectin-3. Hyperplastic pancreatic ductal cells from BOP-treated hamsters heavily expressed galectin-3 (60-95% cells/duct stained). Galectin-3 expression in ductal cells in cancerous pancreatic lesions was increased to >95%. Galectin-3 was also detected in the pancreatic nerves in all human tissue specimens tested.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalInternational Journal of Pancreatology
Volume23
Issue number1
StatePublished - Mar 28 1998

Fingerprint

Galectin 3
Pancreatic Diseases
Cricetinae
nitrosobis(2-oxopropyl)amine
Tumor Cell Line
Monoclonal Antibodies
Neoplasms
Pancreatic Ducts
Chronic Pancreatitis
Pancreatic Neoplasms
Pancreatitis
Paraffin
Membrane Proteins

Keywords

  • Galectin-3
  • Pancreatic cancer
  • Pancreatitis

ASJC Scopus subject areas

  • Oncology
  • Endocrinology
  • Gastroenterology

Cite this

Localization of galectin-3 in normal and diseased pancreatic tissue. / Schaffert, Courtney; Pour, Parviz M.; Chaney, William G.

In: International Journal of Pancreatology, Vol. 23, No. 1, 28.03.1998, p. 1-9.

Research output: Contribution to journalArticle

Schaffert, C, Pour, PM & Chaney, WG 1998, 'Localization of galectin-3 in normal and diseased pancreatic tissue', International Journal of Pancreatology, vol. 23, no. 1, pp. 1-9.
Schaffert, Courtney ; Pour, Parviz M. ; Chaney, William G. / Localization of galectin-3 in normal and diseased pancreatic tissue. In: International Journal of Pancreatology. 1998 ; Vol. 23, No. 1. pp. 1-9.
@article{28c3313a2b2a4eeda78eaff2af39b707,
title = "Localization of galectin-3 in normal and diseased pancreatic tissue",
abstract = "Conclusion. Galectin-3 is expressed in both human and hamster pancreatic tumors and tumor cell lines and this expression is increased over normal. Background. Galectin-3 is overexpressed in many gastrointestinal tumors. This study examined the expression of galectin-3 in human and hamster pancreatic tumors to determine if galectin-3 could be used as a marker for pancreatic cancer. Methods. Membranes were prepared from human and hamster pancreatic tumor cell lines. Galectin-3 was visualized by immunoblot analysis of separated membrane proteins using the monoclonal antibody (MAb) M3/38. Paraffin-embedded sections from normal, pancreatitis, and cancerous human pancreatic tissue and normal, N-nitrosobis(2-oxopropyl)amine (BOP)-treated hyperplastic, and cancerous hamster pancreatic tissues were processed immunohistochemically for galectin-3 using the MAb M3/38. Results. Galectin- 3 was heavily expressed in cytoplasmic and nuclear regions of 50{\%} of normal human pancreatic tissue. Expression of galectin-3 in ductal cells in chronic pancreatitis and cancerous pancreatic tissue was increased over normal and was more uniform (>95{\%} cells/duct stained). Normal hamster pancreatic ducts showed weak or no expression of galectin-3. Hyperplastic pancreatic ductal cells from BOP-treated hamsters heavily expressed galectin-3 (60-95{\%} cells/duct stained). Galectin-3 expression in ductal cells in cancerous pancreatic lesions was increased to >95{\%}. Galectin-3 was also detected in the pancreatic nerves in all human tissue specimens tested.",
keywords = "Galectin-3, Pancreatic cancer, Pancreatitis",
author = "Courtney Schaffert and Pour, {Parviz M.} and Chaney, {William G.}",
year = "1998",
month = "3",
day = "28",
language = "English (US)",
volume = "23",
pages = "1--9",
journal = "Journal of Gastrointestinal Cancer",
issn = "1941-6628",
publisher = "Humana Press",
number = "1",

}

TY - JOUR

T1 - Localization of galectin-3 in normal and diseased pancreatic tissue

AU - Schaffert, Courtney

AU - Pour, Parviz M.

AU - Chaney, William G.

PY - 1998/3/28

Y1 - 1998/3/28

N2 - Conclusion. Galectin-3 is expressed in both human and hamster pancreatic tumors and tumor cell lines and this expression is increased over normal. Background. Galectin-3 is overexpressed in many gastrointestinal tumors. This study examined the expression of galectin-3 in human and hamster pancreatic tumors to determine if galectin-3 could be used as a marker for pancreatic cancer. Methods. Membranes were prepared from human and hamster pancreatic tumor cell lines. Galectin-3 was visualized by immunoblot analysis of separated membrane proteins using the monoclonal antibody (MAb) M3/38. Paraffin-embedded sections from normal, pancreatitis, and cancerous human pancreatic tissue and normal, N-nitrosobis(2-oxopropyl)amine (BOP)-treated hyperplastic, and cancerous hamster pancreatic tissues were processed immunohistochemically for galectin-3 using the MAb M3/38. Results. Galectin- 3 was heavily expressed in cytoplasmic and nuclear regions of 50% of normal human pancreatic tissue. Expression of galectin-3 in ductal cells in chronic pancreatitis and cancerous pancreatic tissue was increased over normal and was more uniform (>95% cells/duct stained). Normal hamster pancreatic ducts showed weak or no expression of galectin-3. Hyperplastic pancreatic ductal cells from BOP-treated hamsters heavily expressed galectin-3 (60-95% cells/duct stained). Galectin-3 expression in ductal cells in cancerous pancreatic lesions was increased to >95%. Galectin-3 was also detected in the pancreatic nerves in all human tissue specimens tested.

AB - Conclusion. Galectin-3 is expressed in both human and hamster pancreatic tumors and tumor cell lines and this expression is increased over normal. Background. Galectin-3 is overexpressed in many gastrointestinal tumors. This study examined the expression of galectin-3 in human and hamster pancreatic tumors to determine if galectin-3 could be used as a marker for pancreatic cancer. Methods. Membranes were prepared from human and hamster pancreatic tumor cell lines. Galectin-3 was visualized by immunoblot analysis of separated membrane proteins using the monoclonal antibody (MAb) M3/38. Paraffin-embedded sections from normal, pancreatitis, and cancerous human pancreatic tissue and normal, N-nitrosobis(2-oxopropyl)amine (BOP)-treated hyperplastic, and cancerous hamster pancreatic tissues were processed immunohistochemically for galectin-3 using the MAb M3/38. Results. Galectin- 3 was heavily expressed in cytoplasmic and nuclear regions of 50% of normal human pancreatic tissue. Expression of galectin-3 in ductal cells in chronic pancreatitis and cancerous pancreatic tissue was increased over normal and was more uniform (>95% cells/duct stained). Normal hamster pancreatic ducts showed weak or no expression of galectin-3. Hyperplastic pancreatic ductal cells from BOP-treated hamsters heavily expressed galectin-3 (60-95% cells/duct stained). Galectin-3 expression in ductal cells in cancerous pancreatic lesions was increased to >95%. Galectin-3 was also detected in the pancreatic nerves in all human tissue specimens tested.

KW - Galectin-3

KW - Pancreatic cancer

KW - Pancreatitis

UR - http://www.scopus.com/inward/record.url?scp=0031886393&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031886393&partnerID=8YFLogxK

M3 - Article

C2 - 9520085

AN - SCOPUS:0031886393

VL - 23

SP - 1

EP - 9

JO - Journal of Gastrointestinal Cancer

JF - Journal of Gastrointestinal Cancer

SN - 1941-6628

IS - 1

ER -