Localization of factor VIII/von willebrand factor and glial fibrillary acidic protein in the hemangioblastoma: Implications for stromal cell histogenesis

R. D. McComb, T. R. Jones, S. V. Pizzo, D. D. Bigner

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The histogenesis of hemangioblastoma stromal cells is unresolved. Ultrastructural observations suggest that the stromal cells, endothelial cells, and pericytes that compose this neoplasm are all derived from angiogenic mesenchyme. The expression of factor VIII/von Willebrand factor (FVIII/vWF), a specific marker for endothelial cells, and of glial fibrillary acidic protein (GFAP), a specific marker for glial cells, was examined in 16 hemangioblastomas using the peroxidase-antiperoxidase immunohistochemical method. Endothelial cell staining for FVIII/vWF was intense in 14 tumors, weak in one, and absent in another. There was no stromal cell staining in any of the neoplasms. Process-bearing, GFAP-positive cells were observed near the tumor margin in 13 cases, and deeper in the neoplasm in 8. In two of these tumors there were also occasional GFAP-positive cells that lacked processes and had a vacuolated cytoplasm. Virtually all of the GFAP-positive cells were interpreted as trapped astrocytes rather than stromal cells. The lack of expression of FVIII/vWF by the stromal cells indicates that they are antigenically distinct from endothelial cells. Several alternatives for stromal cell histogenesis remain open. The stromal cells may be derived from endothelial cells that have undergone antigenic loss, or from angiogenic mesenchymal cells that do not express FVIII/vWF. Alternatively, the stromal cells may originate from nonangiogenic mesenchymal cells derived from the mesoderm or neuroectoderm.

Original languageEnglish (US)
Pages (from-to)207-213
Number of pages7
JournalActa Neuropathologica
Volume56
Issue number3
DOIs
StatePublished - Sep 1 1982

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Hemangioblastoma
Glial Fibrillary Acidic Protein
Stromal Cells
Endothelial Cells
Neoplasms
Mesoderm
Staining and Labeling
Neural Plate
Pericytes
factor VIII, von Willebrand factor drug combination
Neuroglia
Astrocytes
Peroxidase
Cytoplasm

Keywords

  • Endothelial cell
  • Factor VIII/von Willebrand factor
  • Glial fibrillary acidic protein
  • Hemangioblastoma
  • Immunohistochemistry

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

Cite this

Localization of factor VIII/von willebrand factor and glial fibrillary acidic protein in the hemangioblastoma : Implications for stromal cell histogenesis. / McComb, R. D.; Jones, T. R.; Pizzo, S. V.; Bigner, D. D.

In: Acta Neuropathologica, Vol. 56, No. 3, 01.09.1982, p. 207-213.

Research output: Contribution to journalArticle

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