Localization of estrogen receptor regulatory factor in the uterine nucleus

R. G. MacDonald, S. P. Rosenberg, W. W. Leavitt

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The objective of this study was to determine whether progesterone-induced estrogen receptor regulatory factor (ReRF) is a component of hamster uterine nuclei or a cytpplasmic contaminant of the nuclear fraction. Crude uterine nuclei were prepared in low salt buffer and were purified by either the hexylene glycol method or an isotonic sucrose-Triton X-100 procedure. ReRF activity was measured as a net percentage increase in estrogen receptor inactivation after incubation of a 0.5 M KCl nuclear extract at 36°C for 30 min. Progesterone treatment (5 mg/kg s.c. in oil, 2 h) increased net receptor inactivation to the same extent (9-12% net increase) in extract made from each nuclear preparation. Extensive washing of nuclei caused a progressive decline in recovery of ReRF activity, in parallel with the behavior of KCl-extractable nuclear protein and estrogen receptor. These results demonstrate that ReRF is a loosely bound component of the uterine nucleus.

Original languageEnglish (US)
Pages (from-to)301-313
Number of pages13
JournalMolecular and Cellular Endocrinology
Volume32
Issue number2-3
DOIs
StatePublished - Oct 1983

Fingerprint

Estrogen Receptors
Progesterone
Octoxynol
Cytoplasmic and Nuclear Receptors
Nuclear Proteins
Washing
Cricetinae
Sucrose
Buffers
Oils
Salts
Impurities
Recovery
hexylene glycol

Keywords

  • estrogen receptor
  • hamster
  • progesterone induction
  • receptor inactivation
  • uterus

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

Localization of estrogen receptor regulatory factor in the uterine nucleus. / MacDonald, R. G.; Rosenberg, S. P.; Leavitt, W. W.

In: Molecular and Cellular Endocrinology, Vol. 32, No. 2-3, 10.1983, p. 301-313.

Research output: Contribution to journalArticle

MacDonald, R. G. ; Rosenberg, S. P. ; Leavitt, W. W. / Localization of estrogen receptor regulatory factor in the uterine nucleus. In: Molecular and Cellular Endocrinology. 1983 ; Vol. 32, No. 2-3. pp. 301-313.
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