Localization of aldose reductase in the human eye

Y. Akagi, Y. Yajima, P. F. Kador, T. Kuwabara, J. H. Kinoshita

Research output: Contribution to journalArticle

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Abstract

The presence of the enzyme aldose reductase is increasingly being linked to diabetic complications. The distribution of this enzyme in human cornea, lens, retina, and optic nerve has been studied using specific antibodies against purified human placental aldose reductase raised in both rabbit and goat. The antisera from both animals gave equal, specific reactions. In frozen sections of ocular tissues, significant aldose reductase localization was reproducibly demonstrated in the endothelium and epithelium of the cornea and in the basal cell layers of the conjunctiva. In the lens, staining was observed in the epithelium and superficial lens fibers. In retinal sections, the presence of aldose reductase was demonstrated in the Mueller's cells, especially near the inner limiting membrane. It was also found in some ganglion and cone cells. In the optic nerve, positive staining was observed in the axon. All other cells of the tissues examined revealed only weak, nonspecific staining.

Original languageEnglish (US)
Pages (from-to)562-566
Number of pages5
JournalDiabetes
Volume33
Issue number6
DOIs
StatePublished - Jan 1 1984

Fingerprint

Aldehyde Reductase
Lenses
Staining and Labeling
Optic Nerve
Cornea
Epithelium
Conjunctiva
Frozen Sections
Diabetes Complications
Enzymes
Goats
Ganglia
Endothelium
Axons
Retina
Immune Sera
Rabbits
Membranes
Antibodies

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Akagi, Y., Yajima, Y., Kador, P. F., Kuwabara, T., & Kinoshita, J. H. (1984). Localization of aldose reductase in the human eye. Diabetes, 33(6), 562-566. https://doi.org/10.2337/diab.33.6.562

Localization of aldose reductase in the human eye. / Akagi, Y.; Yajima, Y.; Kador, P. F.; Kuwabara, T.; Kinoshita, J. H.

In: Diabetes, Vol. 33, No. 6, 01.01.1984, p. 562-566.

Research output: Contribution to journalArticle

Akagi, Y, Yajima, Y, Kador, PF, Kuwabara, T & Kinoshita, JH 1984, 'Localization of aldose reductase in the human eye', Diabetes, vol. 33, no. 6, pp. 562-566. https://doi.org/10.2337/diab.33.6.562
Akagi Y, Yajima Y, Kador PF, Kuwabara T, Kinoshita JH. Localization of aldose reductase in the human eye. Diabetes. 1984 Jan 1;33(6):562-566. https://doi.org/10.2337/diab.33.6.562
Akagi, Y. ; Yajima, Y. ; Kador, P. F. ; Kuwabara, T. ; Kinoshita, J. H. / Localization of aldose reductase in the human eye. In: Diabetes. 1984 ; Vol. 33, No. 6. pp. 562-566.
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