Local muscle cooling does not impact expression of mitochondrial-related genes

Robert Shute, Matthew Heesch, Terence Laursen, Dustin Slivka

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Recovery that takes place in a cold environment after endurance exercise elevates PGC-1α mRNA whereas ERRα and NRF2 mRNA expression are inhibited. However, the effect of local skeletal muscle cooling on mitochondrial-related gene expression is unknown. Purpose To determine the impact of local skeletal muscle cooling during recovery from an acute bout of exercise on mitochondrial-related gene expression. Methods Recreationally-trained male cyclists (n=8, age 25±3 y, height 181±6 cm, weight 79±8 kg, 12.8±3.6% body fat, VO2peak 4.52±0.88 L·min−1 protocol) completed a 90-min variable intensity cycling protocol followed by 4 h of recovery. During recovery, ice was applied intermittently to one leg (ICE) while the other leg served as a control (CON). Intramuscular temperature was recorded continuously. Muscle biopsies were taken from each vastus lateralis at 4 h post-exercise for the analysis of mitochondrial-related gene expression. Results Intramuscular temperature was colder in ICE (26.7±1.1 °C) than CON (35.5±0.1 °C) throughout the 4 h recovery period (p<0.001). There were no differences in expression of PGC-1α, TFAM, NRF1, NRF2, or ERRα mRNA between ICE and CON after the 4 h recovery period. Conclusion Local muscle cooling after exercise does not impact the expression of mitochondrial biogenesis-related genes compared to recovery from exercise in control conditions. When these data are considered with previous research, the stimuli for cold-induced gene expression alterations may be related to factors other than local muscle temperature. Additionally, different intramuscular temperatures should be examined to determine dose-response of mitochondrial-related gene expression.

    Original languageEnglish (US)
    Pages (from-to)35-39
    Number of pages5
    JournalJournal of Thermal Biology
    Volume67
    DOIs
    StatePublished - Jul 1 2017

    Fingerprint

    Mitochondrial Genes
    Muscle
    exercise
    cooling
    Genes
    Gene expression
    Cooling
    Gene Expression
    Recovery
    Muscles
    gene expression
    muscles
    genes
    Messenger RNA
    Temperature
    skeletal muscle
    Leg
    legs
    temperature
    Skeletal Muscle

    Keywords

    • Exercise
    • Ice
    • PGC-1alpha
    • Vastus Lateralis

    ASJC Scopus subject areas

    • Physiology
    • Biochemistry
    • Agricultural and Biological Sciences(all)
    • Developmental Biology

    Cite this

    Local muscle cooling does not impact expression of mitochondrial-related genes. / Shute, Robert; Heesch, Matthew; Laursen, Terence; Slivka, Dustin.

    In: Journal of Thermal Biology, Vol. 67, 01.07.2017, p. 35-39.

    Research output: Contribution to journalArticle

    Shute, Robert ; Heesch, Matthew ; Laursen, Terence ; Slivka, Dustin. / Local muscle cooling does not impact expression of mitochondrial-related genes. In: Journal of Thermal Biology. 2017 ; Vol. 67. pp. 35-39.
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    abstract = "Recovery that takes place in a cold environment after endurance exercise elevates PGC-1α mRNA whereas ERRα and NRF2 mRNA expression are inhibited. However, the effect of local skeletal muscle cooling on mitochondrial-related gene expression is unknown. Purpose To determine the impact of local skeletal muscle cooling during recovery from an acute bout of exercise on mitochondrial-related gene expression. Methods Recreationally-trained male cyclists (n=8, age 25±3 y, height 181±6 cm, weight 79±8 kg, 12.8±3.6{\%} body fat, VO2peak 4.52±0.88 L·min−1 protocol) completed a 90-min variable intensity cycling protocol followed by 4 h of recovery. During recovery, ice was applied intermittently to one leg (ICE) while the other leg served as a control (CON). Intramuscular temperature was recorded continuously. Muscle biopsies were taken from each vastus lateralis at 4 h post-exercise for the analysis of mitochondrial-related gene expression. Results Intramuscular temperature was colder in ICE (26.7±1.1 °C) than CON (35.5±0.1 °C) throughout the 4 h recovery period (p<0.001). There were no differences in expression of PGC-1α, TFAM, NRF1, NRF2, or ERRα mRNA between ICE and CON after the 4 h recovery period. Conclusion Local muscle cooling after exercise does not impact the expression of mitochondrial biogenesis-related genes compared to recovery from exercise in control conditions. When these data are considered with previous research, the stimuli for cold-induced gene expression alterations may be related to factors other than local muscle temperature. Additionally, different intramuscular temperatures should be examined to determine dose-response of mitochondrial-related gene expression.",
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    T1 - Local muscle cooling does not impact expression of mitochondrial-related genes

    AU - Shute, Robert

    AU - Heesch, Matthew

    AU - Laursen, Terence

    AU - Slivka, Dustin

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    N2 - Recovery that takes place in a cold environment after endurance exercise elevates PGC-1α mRNA whereas ERRα and NRF2 mRNA expression are inhibited. However, the effect of local skeletal muscle cooling on mitochondrial-related gene expression is unknown. Purpose To determine the impact of local skeletal muscle cooling during recovery from an acute bout of exercise on mitochondrial-related gene expression. Methods Recreationally-trained male cyclists (n=8, age 25±3 y, height 181±6 cm, weight 79±8 kg, 12.8±3.6% body fat, VO2peak 4.52±0.88 L·min−1 protocol) completed a 90-min variable intensity cycling protocol followed by 4 h of recovery. During recovery, ice was applied intermittently to one leg (ICE) while the other leg served as a control (CON). Intramuscular temperature was recorded continuously. Muscle biopsies were taken from each vastus lateralis at 4 h post-exercise for the analysis of mitochondrial-related gene expression. Results Intramuscular temperature was colder in ICE (26.7±1.1 °C) than CON (35.5±0.1 °C) throughout the 4 h recovery period (p<0.001). There were no differences in expression of PGC-1α, TFAM, NRF1, NRF2, or ERRα mRNA between ICE and CON after the 4 h recovery period. Conclusion Local muscle cooling after exercise does not impact the expression of mitochondrial biogenesis-related genes compared to recovery from exercise in control conditions. When these data are considered with previous research, the stimuli for cold-induced gene expression alterations may be related to factors other than local muscle temperature. Additionally, different intramuscular temperatures should be examined to determine dose-response of mitochondrial-related gene expression.

    AB - Recovery that takes place in a cold environment after endurance exercise elevates PGC-1α mRNA whereas ERRα and NRF2 mRNA expression are inhibited. However, the effect of local skeletal muscle cooling on mitochondrial-related gene expression is unknown. Purpose To determine the impact of local skeletal muscle cooling during recovery from an acute bout of exercise on mitochondrial-related gene expression. Methods Recreationally-trained male cyclists (n=8, age 25±3 y, height 181±6 cm, weight 79±8 kg, 12.8±3.6% body fat, VO2peak 4.52±0.88 L·min−1 protocol) completed a 90-min variable intensity cycling protocol followed by 4 h of recovery. During recovery, ice was applied intermittently to one leg (ICE) while the other leg served as a control (CON). Intramuscular temperature was recorded continuously. Muscle biopsies were taken from each vastus lateralis at 4 h post-exercise for the analysis of mitochondrial-related gene expression. Results Intramuscular temperature was colder in ICE (26.7±1.1 °C) than CON (35.5±0.1 °C) throughout the 4 h recovery period (p<0.001). There were no differences in expression of PGC-1α, TFAM, NRF1, NRF2, or ERRα mRNA between ICE and CON after the 4 h recovery period. Conclusion Local muscle cooling after exercise does not impact the expression of mitochondrial biogenesis-related genes compared to recovery from exercise in control conditions. When these data are considered with previous research, the stimuli for cold-induced gene expression alterations may be related to factors other than local muscle temperature. Additionally, different intramuscular temperatures should be examined to determine dose-response of mitochondrial-related gene expression.

    KW - Exercise

    KW - Ice

    KW - PGC-1alpha

    KW - Vastus Lateralis

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