LN-3: a diagnostic adjunct in cutaneous graft-versus-host disease.

M. S. Synovec, S. W. Braddock, J. Jones, James Linder

Research output: Contribution to journalArticle

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Abstract

Expression of HLA Class II antigen on keratinocytes has been advocated as a diagnostic marker of acute graft-versus-host disease (GVHD). LN-3 is a murine monoclonal antibody marking an HLA Class II antigen that survives formalin fixation and paraffin embedding. We analyzed the LN-3 staining pattern on 56 skin biopsies from patients treated with bone marrow or peripheral stem cell transplantation, non-transplant patients receiving conventional chemotherapy and/or radiation therapy and controls. Keratinocyte staining, endothelial staining, and the number of epidermal and dermal Langerhans cells were evaluated and analyzed with respect to the histologic and clinical diagnosis in a blind and retrospective fashion. The most predictive parameter for GVHD was marking of endothelial cells by LN-3 which was present in 12 of 16 (75%) biopsies from patients with GVHD. Endothelial staining was found in a total of 19 biopsies of which 12 (63%) had GVHD. These data suggest that LN-3 immunohistochemistry may help identify cutaneous GVHD and discriminate it from conditions that are histologically similar.

Original languageEnglish (US)
Pages (from-to)643-647
Number of pages5
JournalModern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
Volume3
Issue number6
StatePublished - Nov 1990

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Graft vs Host Disease
Skin
Staining and Labeling
Histocompatibility Antigens Class II
HLA Antigens
Keratinocytes
Biopsy
Paraffin Embedding
Peripheral Blood Stem Cell Transplantation
Langerhans Cells
Formaldehyde
Radiotherapy
Endothelial Cells
Bone Marrow
Immunohistochemistry
Monoclonal Antibodies
Drug Therapy

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

LN-3 : a diagnostic adjunct in cutaneous graft-versus-host disease. / Synovec, M. S.; Braddock, S. W.; Jones, J.; Linder, James.

In: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 3, No. 6, 11.1990, p. 643-647.

Research output: Contribution to journalArticle

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