Liver proteome response of largemouth bass (Micropterus salmoides) exposed to several environmental contaminants

Potential insights into biomarker development

Brian C. Sanchez, Kimberly J. Ralston-Hooper, Kevin A. Kowalski, H. Dorota Inerowicz, Jiri Adamec, María S. Sepúlveda

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Liver proteome response of largemouth bass (Micropterus salmoides) exposed to environmental contaminants was analyzed to identify novel biomarkers of exposure. Adult male bass were exposed to cadmium chloride (CdCl2), atrazine, PCB 126, phenanthrene, or toxaphene via intraperitoneal injection with target body burdens of 0.00067, 3.0, 2.5, 50, and 100 μg/g, respectively. After a 96 h exposure, hepatic proteins were separated with two-dimensional gel electrophoresis and differentially expressed proteins (vs. controls) recognized and identified with MALDI-TOF/TOF mass spectrometry. We identified, 30, 18, eight, 19, and five proteins as differentially expressed within the CdCl2, atrazine, PCB 126, phenanthrene, and toxaphene treatments, respectively. Alterations were observed in the expression of proteins associated with cellular ion homeostasis (toxaphene), oxidative stress (phenanthrene, PCB 126), and energy production including glycolysis (CdCl2, atrazine) and ATP synthesis (atrazine). This work supports the further evaluation of several of these proteins as biomarkers of contaminant exposure in fish.

Original languageEnglish (US)
Pages (from-to)52-59
Number of pages8
JournalAquatic Toxicology
Volume95
Issue number1
DOIs
StatePublished - Oct 19 2009

Fingerprint

3,4,5,3',4'-pentachlorobiphenyl
Bass
Micropterus salmoides
atrazine
toxaphene
Atrazine
Proteome
proteome
Toxaphene
phenanthrene
biomarker
biomarkers
Cadmium Chloride
Biomarkers
pollution
liver
protein
pollutant
Liver
PCB

Keywords

  • Atrazine
  • Biomarkers
  • Cadmium
  • Largemouth bass
  • Micropterus salmoides
  • Proteomics

ASJC Scopus subject areas

  • Aquatic Science
  • Health, Toxicology and Mutagenesis

Cite this

Liver proteome response of largemouth bass (Micropterus salmoides) exposed to several environmental contaminants : Potential insights into biomarker development. / Sanchez, Brian C.; Ralston-Hooper, Kimberly J.; Kowalski, Kevin A.; Dorota Inerowicz, H.; Adamec, Jiri; Sepúlveda, María S.

In: Aquatic Toxicology, Vol. 95, No. 1, 19.10.2009, p. 52-59.

Research output: Contribution to journalArticle

Sanchez, Brian C. ; Ralston-Hooper, Kimberly J. ; Kowalski, Kevin A. ; Dorota Inerowicz, H. ; Adamec, Jiri ; Sepúlveda, María S. / Liver proteome response of largemouth bass (Micropterus salmoides) exposed to several environmental contaminants : Potential insights into biomarker development. In: Aquatic Toxicology. 2009 ; Vol. 95, No. 1. pp. 52-59.
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