Listeria monocytogenes Scott A transports glucose by high-affinity and low-affinity glucose transport systems

Corrine Parker, Robert W. Hutkins

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Listeria monocytogenes transported glucose by a high-affinity phosphoenolpyruvate-dependent phosphotransferase system and a low-affinity proton motive force-mediated system. The low-affinity system (K(m) = 2.9 mM) was inhibited by 2-deoxyglucose and 6-deoxyglucose, whereas the high-affinity system (K(m) = 0.11 mM) was inhibited by 2-deoxyglucose and mannose but not 6-deoxyglucose. Cells and vesicles artificially energized with valinomycin transported glucose or 2-deoxyglucose at rates greater than those of de- energized cells, indicating that a membrane potential could drive uptake by the low-affinity system.

Original languageEnglish (US)
Pages (from-to)543-546
Number of pages4
JournalApplied and environmental microbiology
Volume63
Issue number2
StatePublished - Feb 1 1997

Fingerprint

2-deoxyglucose
Deoxyglucose
Listeria monocytogenes
deoxyglucose
glucose
Glucose
vesicle
valinomycin
Valinomycin
Phosphoenolpyruvate
Proton-Motive Force
Mannose
membrane
mannose
membrane potential
Membrane Potentials
cell membranes
phosphotransferases (kinases)
Phosphotransferases
uptake mechanisms

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Listeria monocytogenes Scott A transports glucose by high-affinity and low-affinity glucose transport systems. / Parker, Corrine; Hutkins, Robert W.

In: Applied and environmental microbiology, Vol. 63, No. 2, 01.02.1997, p. 543-546.

Research output: Contribution to journalArticle

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