Liquid chromatography-mass spectrometry method for the analysis of the anti-cancer agent capecitabine and its nucleoside metabolites in human plasma

Yan Xu, Jean L Grem

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

A reversed-phase high-performance liquid chromatography method with electrospray ionization and mass spectral detection is described for the determination of capecitabine, 5′-deoxy-5-fluorocytidine and 5′-deoxy-5-fluorouridine in human plasma with 5-chloro-2′-deoxyuridine as the internal standard. An on-line sample clean-up procedure allows dilution of the plasma sample with the initial mobile phase. The linear dynamic range is 0.0500-10.0 μg/ml for capecitabine, and 0.0500-25.0 μg/ml for the metabolites, 5′-deoxy-5-fluorocytidine and 5′-deoxy-5-fluorouridine, respectively. This method has been used to analyze plasma samples from patients receiving capecitabine in combination with oxaliplatin.

Original languageEnglish (US)
Pages (from-to)273-285
Number of pages13
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume783
Issue number1
DOIs
StatePublished - Jan 5 2003

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Plasma (human)
Liquid chromatography
Metabolites
Nucleosides
Liquid Chromatography
Mass spectrometry
Mass Spectrometry
oxaliplatin
Plasmas
Electrospray ionization
Neoplasms
High performance liquid chromatography
Reverse-Phase Chromatography
Dilution
High Pressure Liquid Chromatography
Capecitabine
doxifluridine
5'-deoxy-5-fluorocytidine

Keywords

  • 5-Deoxy-5-fluorocytidine
  • 5-Deoxy-5-fluorouridine
  • Capecitabine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

Cite this

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abstract = "A reversed-phase high-performance liquid chromatography method with electrospray ionization and mass spectral detection is described for the determination of capecitabine, 5′-deoxy-5-fluorocytidine and 5′-deoxy-5-fluorouridine in human plasma with 5-chloro-2′-deoxyuridine as the internal standard. An on-line sample clean-up procedure allows dilution of the plasma sample with the initial mobile phase. The linear dynamic range is 0.0500-10.0 μg/ml for capecitabine, and 0.0500-25.0 μg/ml for the metabolites, 5′-deoxy-5-fluorocytidine and 5′-deoxy-5-fluorouridine, respectively. This method has been used to analyze plasma samples from patients receiving capecitabine in combination with oxaliplatin.",
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