Liposarcomatous differentiation in malignant phyllodes tumours is unassociated with MDM2 or CDK4 amplification

Pamela L. Lyle, Julia A. Bridge, Jean F. Simpson, Justin M. Cates, Melinda E. Sanders

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Aims: Breast sarcomas are rare, usually occurring in the setting of malignant phyllodes tumour (MPT). Heterologous differentiation commonly resembles well-differentiated or pleomorphic liposarcoma. In extramammary sites, these subtypes have different biological behaviours and distinct genetic alterations: MDM2 and CDK4 amplification in well-differentiated liposarcoma, and polyploidy with complex structural rearrangements in pleomorphic liposarcoma. The aim of this study was to investigate foci resembling well-differentiated liposarcoma in MPT for MDM2 and CDK4 amplification. Methods and results: We evaluated the clinicopathological characteristics of MPTs received by the Vanderbilt Breast Consultation Service containing components resembling well-differentiated or pleomorphic liposarcoma. Cases with available tissue blocks were subjected to fluorescence in-situ hybridization with MDM2 and CDK4 probes. Thirty-eight MPTs with liposarcomatous components were available for review. The mean patient age was 49.8 years (range 26-84 years). In addition to well-differentiated liposarcoma, the following components were also present: high-grade undifferentiated sarcoma (n = 9; 23.7%), pleomorphic liposarcoma (n = 4; 10.5%), non-high-grade sarcoma not otherwise specified (n = 22; 57.9%), and malignant peripheral nerve sheath tumour-like (n = 2; 5.2%). Among 10 cases tested, none showed amplification of MDM2 or CDK4. Conclusions: This study examined molecular changes in the well-differentiated liposarcomatous components of MPT. Despite histological similarity to well-differentiated liposarcoma of soft tissues, liposarcomatous differentiation in MPT lacks the molecular phenotype characteristic of extramammary well-differentiated liposarcoma.

Original languageEnglish (US)
Pages (from-to)1040-1045
Number of pages6
JournalHistopathology
Volume68
Issue number7
DOIs
StatePublished - Jun 1 2016

Fingerprint

Phyllodes Tumor
Liposarcoma
Sarcoma
Breast
Polyploidy
Neurilemmoma
Fluorescence In Situ Hybridization
Referral and Consultation
Phenotype

Keywords

  • CDK4
  • Liposarcoma
  • Liposarcomatous differentiation
  • MDM2
  • Malignant phyllodes tumour

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

Cite this

Lyle, P. L., Bridge, J. A., Simpson, J. F., Cates, J. M., & Sanders, M. E. (2016). Liposarcomatous differentiation in malignant phyllodes tumours is unassociated with MDM2 or CDK4 amplification. Histopathology, 68(7), 1040-1045. https://doi.org/10.1111/his.12898

Liposarcomatous differentiation in malignant phyllodes tumours is unassociated with MDM2 or CDK4 amplification. / Lyle, Pamela L.; Bridge, Julia A.; Simpson, Jean F.; Cates, Justin M.; Sanders, Melinda E.

In: Histopathology, Vol. 68, No. 7, 01.06.2016, p. 1040-1045.

Research output: Contribution to journalArticle

Lyle, Pamela L. ; Bridge, Julia A. ; Simpson, Jean F. ; Cates, Justin M. ; Sanders, Melinda E. / Liposarcomatous differentiation in malignant phyllodes tumours is unassociated with MDM2 or CDK4 amplification. In: Histopathology. 2016 ; Vol. 68, No. 7. pp. 1040-1045.
@article{ea2d31dd97554111a9b749c18b2f116e,
title = "Liposarcomatous differentiation in malignant phyllodes tumours is unassociated with MDM2 or CDK4 amplification",
abstract = "Aims: Breast sarcomas are rare, usually occurring in the setting of malignant phyllodes tumour (MPT). Heterologous differentiation commonly resembles well-differentiated or pleomorphic liposarcoma. In extramammary sites, these subtypes have different biological behaviours and distinct genetic alterations: MDM2 and CDK4 amplification in well-differentiated liposarcoma, and polyploidy with complex structural rearrangements in pleomorphic liposarcoma. The aim of this study was to investigate foci resembling well-differentiated liposarcoma in MPT for MDM2 and CDK4 amplification. Methods and results: We evaluated the clinicopathological characteristics of MPTs received by the Vanderbilt Breast Consultation Service containing components resembling well-differentiated or pleomorphic liposarcoma. Cases with available tissue blocks were subjected to fluorescence in-situ hybridization with MDM2 and CDK4 probes. Thirty-eight MPTs with liposarcomatous components were available for review. The mean patient age was 49.8 years (range 26-84 years). In addition to well-differentiated liposarcoma, the following components were also present: high-grade undifferentiated sarcoma (n = 9; 23.7{\%}), pleomorphic liposarcoma (n = 4; 10.5{\%}), non-high-grade sarcoma not otherwise specified (n = 22; 57.9{\%}), and malignant peripheral nerve sheath tumour-like (n = 2; 5.2{\%}). Among 10 cases tested, none showed amplification of MDM2 or CDK4. Conclusions: This study examined molecular changes in the well-differentiated liposarcomatous components of MPT. Despite histological similarity to well-differentiated liposarcoma of soft tissues, liposarcomatous differentiation in MPT lacks the molecular phenotype characteristic of extramammary well-differentiated liposarcoma.",
keywords = "CDK4, Liposarcoma, Liposarcomatous differentiation, MDM2, Malignant phyllodes tumour",
author = "Lyle, {Pamela L.} and Bridge, {Julia A.} and Simpson, {Jean F.} and Cates, {Justin M.} and Sanders, {Melinda E.}",
year = "2016",
month = "6",
day = "1",
doi = "10.1111/his.12898",
language = "English (US)",
volume = "68",
pages = "1040--1045",
journal = "Histopathology",
issn = "0309-0167",
publisher = "Wiley-Blackwell",
number = "7",

}

TY - JOUR

T1 - Liposarcomatous differentiation in malignant phyllodes tumours is unassociated with MDM2 or CDK4 amplification

AU - Lyle, Pamela L.

AU - Bridge, Julia A.

AU - Simpson, Jean F.

AU - Cates, Justin M.

AU - Sanders, Melinda E.

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Aims: Breast sarcomas are rare, usually occurring in the setting of malignant phyllodes tumour (MPT). Heterologous differentiation commonly resembles well-differentiated or pleomorphic liposarcoma. In extramammary sites, these subtypes have different biological behaviours and distinct genetic alterations: MDM2 and CDK4 amplification in well-differentiated liposarcoma, and polyploidy with complex structural rearrangements in pleomorphic liposarcoma. The aim of this study was to investigate foci resembling well-differentiated liposarcoma in MPT for MDM2 and CDK4 amplification. Methods and results: We evaluated the clinicopathological characteristics of MPTs received by the Vanderbilt Breast Consultation Service containing components resembling well-differentiated or pleomorphic liposarcoma. Cases with available tissue blocks were subjected to fluorescence in-situ hybridization with MDM2 and CDK4 probes. Thirty-eight MPTs with liposarcomatous components were available for review. The mean patient age was 49.8 years (range 26-84 years). In addition to well-differentiated liposarcoma, the following components were also present: high-grade undifferentiated sarcoma (n = 9; 23.7%), pleomorphic liposarcoma (n = 4; 10.5%), non-high-grade sarcoma not otherwise specified (n = 22; 57.9%), and malignant peripheral nerve sheath tumour-like (n = 2; 5.2%). Among 10 cases tested, none showed amplification of MDM2 or CDK4. Conclusions: This study examined molecular changes in the well-differentiated liposarcomatous components of MPT. Despite histological similarity to well-differentiated liposarcoma of soft tissues, liposarcomatous differentiation in MPT lacks the molecular phenotype characteristic of extramammary well-differentiated liposarcoma.

AB - Aims: Breast sarcomas are rare, usually occurring in the setting of malignant phyllodes tumour (MPT). Heterologous differentiation commonly resembles well-differentiated or pleomorphic liposarcoma. In extramammary sites, these subtypes have different biological behaviours and distinct genetic alterations: MDM2 and CDK4 amplification in well-differentiated liposarcoma, and polyploidy with complex structural rearrangements in pleomorphic liposarcoma. The aim of this study was to investigate foci resembling well-differentiated liposarcoma in MPT for MDM2 and CDK4 amplification. Methods and results: We evaluated the clinicopathological characteristics of MPTs received by the Vanderbilt Breast Consultation Service containing components resembling well-differentiated or pleomorphic liposarcoma. Cases with available tissue blocks were subjected to fluorescence in-situ hybridization with MDM2 and CDK4 probes. Thirty-eight MPTs with liposarcomatous components were available for review. The mean patient age was 49.8 years (range 26-84 years). In addition to well-differentiated liposarcoma, the following components were also present: high-grade undifferentiated sarcoma (n = 9; 23.7%), pleomorphic liposarcoma (n = 4; 10.5%), non-high-grade sarcoma not otherwise specified (n = 22; 57.9%), and malignant peripheral nerve sheath tumour-like (n = 2; 5.2%). Among 10 cases tested, none showed amplification of MDM2 or CDK4. Conclusions: This study examined molecular changes in the well-differentiated liposarcomatous components of MPT. Despite histological similarity to well-differentiated liposarcoma of soft tissues, liposarcomatous differentiation in MPT lacks the molecular phenotype characteristic of extramammary well-differentiated liposarcoma.

KW - CDK4

KW - Liposarcoma

KW - Liposarcomatous differentiation

KW - MDM2

KW - Malignant phyllodes tumour

UR - http://www.scopus.com/inward/record.url?scp=84964703628&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84964703628&partnerID=8YFLogxK

U2 - 10.1111/his.12898

DO - 10.1111/his.12898

M3 - Article

C2 - 26542423

AN - SCOPUS:84964703628

VL - 68

SP - 1040

EP - 1045

JO - Histopathology

JF - Histopathology

SN - 0309-0167

IS - 7

ER -