Lipoprotein receptors in copper-deficient rats

Apolipoprotein E-free high density lipoprotein binding to liver membranes

C. A. Hassel, K. Y. Lei, Timothy P Carr, J. A. Marchello

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Twenty-four male weanling Sprague-Dawley rats were randomly and equally divided into two dietary treatments, copper-deficient and adequate (0.7 mg and 8.0 mg Cu/kg diet, respectively). Deionized water and diet were provided ad libitum. After 8 weeks, rats were exsanguinated, membranes prepared from livers, and plasma high density lipoproteins (HDL) isolated by ultracentrifugation and agarose column chromatography. Heparin-sepharose affinity chromatography was used to isolate subfractions of HDL devoid of apolipoprotein E (apo E-free HDL). The apo-E-free HDL derived from rats of each dietary treatment were iodinated and bound to liver membranes prepared from rats of both treatment groups. Total binding data, specific binding data, and computer derived estimates of maximum equilibrium binding (Bmax) indicate less binding was observed when lipoproteins and membranes from copper-deficient animals were used in the binding assay compared to controls. In addition, a 2 × 2 factorial analysis of binding parameters derived from all experiments demonstrated a significant lipoprotein effect, indicating the reduction in binding may be associated with apo E-free HDL obtained from copper-deficient rats. The present findings suggest a reduction in binding of apo E-free HDL to their binding sites may contribute to the hypercholesterolemia and hyperlipoproteinemia observed in copper deficiency.

Original languageEnglish (US)
Pages (from-to)1054-1062
Number of pages9
JournalMetabolism
Volume36
Issue number11
DOIs
StatePublished - Jan 1 1987

Fingerprint

Lipoprotein Receptors
HDL Lipoproteins
Copper
Apolipoproteins E
Membranes
Liver
Agarose Chromatography
Lipoproteins
Diet
Hyperlipoproteinemias
Ultracentrifugation
Hypercholesterolemia
Affinity Chromatography
Sprague Dawley Rats
Binding Sites
apolipoprotein E-rich HDL
Water

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Lipoprotein receptors in copper-deficient rats : Apolipoprotein E-free high density lipoprotein binding to liver membranes. / Hassel, C. A.; Lei, K. Y.; Carr, Timothy P; Marchello, J. A.

In: Metabolism, Vol. 36, No. 11, 01.01.1987, p. 1054-1062.

Research output: Contribution to journalArticle

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