Lipoic acid (thioctic acid) analogs, tryptophan analogs, and urea do not interfere with the assay of biotin and biotin metabolites by high-performance liquid chromatography/avidin-binding assay

Janos Zempleni, Donald B. McCormick, Shawna L. Stratton, Donald M. Mock

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Lipoic acid, urea, and tryptophan show structural similarities to the vitamin biotin. If these compounds can successfully compete with biotin or a biotinylated protein for binding to avidin, their presence in serum and urine would cause artifacts in avidin-binding assays for biotin. We assessed the ability of lipoic acid and certain analogs, urea, and L-tryptophan and tryptophan derivatives to interfere with the measurement of 16 biotin analogs by a high-performance liquid chromotography (HPLC)/avidin-binding assay. In this assay, compounds are separated by reversed-phase HPLC, followed by assay of each fraction based on binding to avidin horseradish peroxidase. At physiologic concentrations, neither lipoic acid analogs (d-lipoate, l-lipoate, d,l-lipoamide, bisnorlipoate, β-hydroxybisnorlipoate, and tetranorlipoate) nor tryptophan derivatives exhibited detectable avidin-binding. Minor avidin-binding was seen for urea and L-tryptophan; binding ratios relative to biotin were 1 x 10-9 and 3 x 10-6, respectively. Urea did not co-elute on HPLC with any of the 16 biotin analogs, but L-tryptophan did co-elute with bisnorbiotin methyl ketone. However, because the relative avidin affinity of L-tryptophan is five orders of magnitude smaller than that of bisnorbiotin methyl ketone, we conclude that none of the tested compounds are likely to interfere with the measurement of biotin or its metabolites by an HPLC/avidin-binding assay.

Original languageEnglish (US)
Pages (from-to)518-523
Number of pages6
JournalJournal of Nutritional Biochemistry
Volume7
Issue number9
DOIs
StatePublished - Sep 1996

Fingerprint

Thioctic Acid
Avidin
High performance liquid chromatography
Biotin
Metabolites
Tryptophan
Urea
Assays
High Pressure Liquid Chromatography
Liquids
Derivatives
Horseradish Peroxidase
Protein Binding
Vitamins
Artifacts
Urine

Keywords

  • avidin-binding assay
  • biotin
  • high-performance liquid chromatography
  • lipoic acid
  • tryptophan
  • urea

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Nutrition and Dietetics
  • Clinical Biochemistry

Cite this

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title = "Lipoic acid (thioctic acid) analogs, tryptophan analogs, and urea do not interfere with the assay of biotin and biotin metabolites by high-performance liquid chromatography/avidin-binding assay",
abstract = "Lipoic acid, urea, and tryptophan show structural similarities to the vitamin biotin. If these compounds can successfully compete with biotin or a biotinylated protein for binding to avidin, their presence in serum and urine would cause artifacts in avidin-binding assays for biotin. We assessed the ability of lipoic acid and certain analogs, urea, and L-tryptophan and tryptophan derivatives to interfere with the measurement of 16 biotin analogs by a high-performance liquid chromotography (HPLC)/avidin-binding assay. In this assay, compounds are separated by reversed-phase HPLC, followed by assay of each fraction based on binding to avidin horseradish peroxidase. At physiologic concentrations, neither lipoic acid analogs (d-lipoate, l-lipoate, d,l-lipoamide, bisnorlipoate, β-hydroxybisnorlipoate, and tetranorlipoate) nor tryptophan derivatives exhibited detectable avidin-binding. Minor avidin-binding was seen for urea and L-tryptophan; binding ratios relative to biotin were 1 x 10-9 and 3 x 10-6, respectively. Urea did not co-elute on HPLC with any of the 16 biotin analogs, but L-tryptophan did co-elute with bisnorbiotin methyl ketone. However, because the relative avidin affinity of L-tryptophan is five orders of magnitude smaller than that of bisnorbiotin methyl ketone, we conclude that none of the tested compounds are likely to interfere with the measurement of biotin or its metabolites by an HPLC/avidin-binding assay.",
keywords = "avidin-binding assay, biotin, high-performance liquid chromatography, lipoic acid, tryptophan, urea",
author = "Janos Zempleni and McCormick, {Donald B.} and Stratton, {Shawna L.} and Mock, {Donald M.}",
year = "1996",
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T1 - Lipoic acid (thioctic acid) analogs, tryptophan analogs, and urea do not interfere with the assay of biotin and biotin metabolites by high-performance liquid chromatography/avidin-binding assay

AU - Zempleni, Janos

AU - McCormick, Donald B.

AU - Stratton, Shawna L.

AU - Mock, Donald M.

PY - 1996/9

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N2 - Lipoic acid, urea, and tryptophan show structural similarities to the vitamin biotin. If these compounds can successfully compete with biotin or a biotinylated protein for binding to avidin, their presence in serum and urine would cause artifacts in avidin-binding assays for biotin. We assessed the ability of lipoic acid and certain analogs, urea, and L-tryptophan and tryptophan derivatives to interfere with the measurement of 16 biotin analogs by a high-performance liquid chromotography (HPLC)/avidin-binding assay. In this assay, compounds are separated by reversed-phase HPLC, followed by assay of each fraction based on binding to avidin horseradish peroxidase. At physiologic concentrations, neither lipoic acid analogs (d-lipoate, l-lipoate, d,l-lipoamide, bisnorlipoate, β-hydroxybisnorlipoate, and tetranorlipoate) nor tryptophan derivatives exhibited detectable avidin-binding. Minor avidin-binding was seen for urea and L-tryptophan; binding ratios relative to biotin were 1 x 10-9 and 3 x 10-6, respectively. Urea did not co-elute on HPLC with any of the 16 biotin analogs, but L-tryptophan did co-elute with bisnorbiotin methyl ketone. However, because the relative avidin affinity of L-tryptophan is five orders of magnitude smaller than that of bisnorbiotin methyl ketone, we conclude that none of the tested compounds are likely to interfere with the measurement of biotin or its metabolites by an HPLC/avidin-binding assay.

AB - Lipoic acid, urea, and tryptophan show structural similarities to the vitamin biotin. If these compounds can successfully compete with biotin or a biotinylated protein for binding to avidin, their presence in serum and urine would cause artifacts in avidin-binding assays for biotin. We assessed the ability of lipoic acid and certain analogs, urea, and L-tryptophan and tryptophan derivatives to interfere with the measurement of 16 biotin analogs by a high-performance liquid chromotography (HPLC)/avidin-binding assay. In this assay, compounds are separated by reversed-phase HPLC, followed by assay of each fraction based on binding to avidin horseradish peroxidase. At physiologic concentrations, neither lipoic acid analogs (d-lipoate, l-lipoate, d,l-lipoamide, bisnorlipoate, β-hydroxybisnorlipoate, and tetranorlipoate) nor tryptophan derivatives exhibited detectable avidin-binding. Minor avidin-binding was seen for urea and L-tryptophan; binding ratios relative to biotin were 1 x 10-9 and 3 x 10-6, respectively. Urea did not co-elute on HPLC with any of the 16 biotin analogs, but L-tryptophan did co-elute with bisnorbiotin methyl ketone. However, because the relative avidin affinity of L-tryptophan is five orders of magnitude smaller than that of bisnorbiotin methyl ketone, we conclude that none of the tested compounds are likely to interfere with the measurement of biotin or its metabolites by an HPLC/avidin-binding assay.

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