LincRNA-Cox2 promotes late inflammatory gene transcription in macrophages through modulating SWI/SNF-mediated chromatin remodeling

Guoku Hu, Ai Yu Gong, Yang Wang, Shibin Ma, Xiqiang Chen, Jing Chen, Chun Jen Su, Annemarie Shibata, Juliane K. Strauss-Soukup, Kristen M. Drescher, Xian Ming Chen

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Long intergenic noncoding RNAs (lincRNAs) are long noncoding transcripts (>200 nt) from the intergenic regions of annotated protein-coding genes. One of the most highly induced lincRNAs in macrophages upon TLR ligation is lincRNA-Cox2, which was recently shown to mediate the activation and repression of distinct classes of immune genes in innate immune cells.We report that lincRNA-Cox2, located at chromosome 1 proximal to the PG-endoperoxide synthase 2 (Ptgs2/Cox2) gene, is an early-primary inflammatory gene controlled by NF-κB signaling in murine macrophages. Functionally, lincRNA-Cox2 is required for the transcription of NF-κB-regulated late-primary inflammatory response genes stimulated by bacterial LPS. Specifically, lincRNA-Cox2 is assembled into the switch/sucrose nonfermentable (SWI/SNF) complex in cells after LPS stimulation. This resulting lincRNA-Cox2/SWI/SNF complex can modulate the assembly of NF-κB subunits to the SWI/SNF complex, and ultimately, SWI/SNF-associated chromatin remodeling and transactivation of the late-primary inflammatory-response genes in macrophages in response to microbial challenge. Therefore, our data indicate a new regulatory role for NF-κB-induced lincRNA-Cox2 as a coactivator of NF-κB for the transcription of late-primary response genes in innate immune cells through modulation of epigenetic chromatin remodeling.

Original languageEnglish (US)
Pages (from-to)2799-2808
Number of pages10
JournalJournal of Immunology
Volume196
Issue number6
DOIs
StatePublished - Mar 15 2016

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Genetic Transcription
Long Noncoding RNA
Chromatin Assembly and Disassembly
Sucrose
Macrophages
Genes
Switch Genes
Bacterial Genes
Intergenic DNA
Chromosomes, Human, Pair 1
Epigenomics
Transcriptional Activation
Ligation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

LincRNA-Cox2 promotes late inflammatory gene transcription in macrophages through modulating SWI/SNF-mediated chromatin remodeling. / Hu, Guoku; Gong, Ai Yu; Wang, Yang; Ma, Shibin; Chen, Xiqiang; Chen, Jing; Su, Chun Jen; Shibata, Annemarie; Strauss-Soukup, Juliane K.; Drescher, Kristen M.; Chen, Xian Ming.

In: Journal of Immunology, Vol. 196, No. 6, 15.03.2016, p. 2799-2808.

Research output: Contribution to journalArticle

Hu, G, Gong, AY, Wang, Y, Ma, S, Chen, X, Chen, J, Su, CJ, Shibata, A, Strauss-Soukup, JK, Drescher, KM & Chen, XM 2016, 'LincRNA-Cox2 promotes late inflammatory gene transcription in macrophages through modulating SWI/SNF-mediated chromatin remodeling', Journal of Immunology, vol. 196, no. 6, pp. 2799-2808. https://doi.org/10.4049/jimmunol.1502146
Hu, Guoku ; Gong, Ai Yu ; Wang, Yang ; Ma, Shibin ; Chen, Xiqiang ; Chen, Jing ; Su, Chun Jen ; Shibata, Annemarie ; Strauss-Soukup, Juliane K. ; Drescher, Kristen M. ; Chen, Xian Ming. / LincRNA-Cox2 promotes late inflammatory gene transcription in macrophages through modulating SWI/SNF-mediated chromatin remodeling. In: Journal of Immunology. 2016 ; Vol. 196, No. 6. pp. 2799-2808.
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AU - Su, Chun Jen

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