Limited Efficacy of Antibacterial Vaccination Against Secondary Serotype 3 Pneumococcal Pneumonia Following Influenza Infection

Dennis W. Metzger, Yoichi Furuya, Sharon L. Salmon, Sean Roberts, Keer Sun

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background. Secondary bacterial infections following influenza represent a major cause of mortality in the human population, which, in turn, has led to a call for stockpiling of bacterial vaccines for pandemic preparedness. Methods. To investigate the efficacy of bacterial vaccination for protection against secondary pneumococcal infection, mice were immunized with pneumococcal capsular polysaccharide conjugate vaccine, and then sequentially coinfected 5 weeks later with PR8 influenza virus and A66.1 Streptococcus pneumoniae. Results. In the absence of influenza virus exposure, vaccination with polysaccharide conjugate vaccine was highly effective, as indicated by 100% survival from lethal pneumococcal pneumonia and 10 000-fold greater efficiency in clearance of bacteria from the lung compared to unvaccinated mice. Enhanced clearance after vaccination was dependent upon Fc receptor (FcR) expression. However, following influenza, <40% of vaccinated mice survived bacterial coinfection and FcR-dependent clearance of antibody-opsonized bacteria reduced bacterial levels in the lungs only 5-10 fold. No differences in lung myeloid cell numbers or in FcR cell surface expression were observed following influenza. Conclusions. The results show that induction of antibacterial humoral immunity is only partially effective in protection against secondary bacterial infections that occur following influenza, and suggest that additional therapeutic strategies to overcome defective antibacterial immunity should be explored.

Original languageEnglish (US)
Pages (from-to)445-452
Number of pages8
JournalJournal of Infectious Diseases
Volume212
Issue number3
DOIs
StatePublished - Aug 1 2015

Fingerprint

Pneumococcal Pneumonia
Coinfection
Human Influenza
Fc Receptors
Vaccination
Conjugate Vaccines
Infection
Orthomyxoviridae
Bacterial Infections
Lung
Polysaccharides
Bacterial Vaccines
Bacteria
Pneumococcal Infections
Pandemics
Myeloid Cells
Humoral Immunity
Streptococcus pneumoniae
Immunity
Cell Count

Keywords

  • adaptive immunity
  • coinfection
  • influenza virus
  • innate immunity
  • pneumococcus
  • pneumonia
  • vaccination

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Limited Efficacy of Antibacterial Vaccination Against Secondary Serotype 3 Pneumococcal Pneumonia Following Influenza Infection. / Metzger, Dennis W.; Furuya, Yoichi; Salmon, Sharon L.; Roberts, Sean; Sun, Keer.

In: Journal of Infectious Diseases, Vol. 212, No. 3, 01.08.2015, p. 445-452.

Research output: Contribution to journalArticle

Metzger, Dennis W. ; Furuya, Yoichi ; Salmon, Sharon L. ; Roberts, Sean ; Sun, Keer. / Limited Efficacy of Antibacterial Vaccination Against Secondary Serotype 3 Pneumococcal Pneumonia Following Influenza Infection. In: Journal of Infectious Diseases. 2015 ; Vol. 212, No. 3. pp. 445-452.
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