LIMD1 phosphorylation in mitosis is required for mitotic progression and its tumor-suppressing activity

Jiuli Zhou, Lin Zhang, Wei Zhou, Yuanhong Chen, Yufeng Cheng, Jixin Dong

Research output: Contribution to journalArticle

Abstract

LIM domains containing 1 (LIMD1) is a member of the Zyxin family proteins and functions as a tumor suppressor in lung cancer. LIMD1 has been shown to regulate Hippo-YAP signaling activity. Here, we report a novel regulatory mechanism for LIMD1. We found that cyclin-dependent kinase 1 (CDK1) and c-Jun NH2-terminal kinases 1/2 (JNK1/2) phosphorylate LIMD1 in vitro and in cells during anti-tubulin drug-induced mitotic arrest. Phosphorylation also occurs during normal mitosis. S272, S277, S421, and S424 were identified as the main phosphorylation sites in LIMD1. Deletion of LIMD1 resulted in a shortened mitotic cell cycle and phosphorylation of LIMD1 is required for proper mitotic progression. We further showed that the phosphorylation-deficient mutant LIMD1-4A is less active in suppressing cell proliferation, anchorage-independent growth, cell migration, and invasion in lung cancer cells. Together, our findings suggest that LIMD1 is a key regulator of mitotic progression, and that dysregulation of LIMD1 contributes to tumorigenesis.

Original languageEnglish (US)
Pages (from-to)963-974
Number of pages12
JournalFEBS Journal
Volume286
Issue number5
DOIs
StatePublished - Mar 1 2019

Fingerprint

Phosphorylation
Mitosis
Tumors
bis(2,3,3,3-tetrachloropropyl) ether
Lung Neoplasms
Neoplasms
Zyxin
Cells
CDC2 Protein Kinase
JNK Mitogen-Activated Protein Kinases
Cell proliferation
Cell growth
Tubulin
Cell Movement
Cell Cycle
Carcinogenesis
Cell Proliferation
Growth
Pharmaceutical Preparations
Proteins

Keywords

  • CDK1
  • Hippo
  • JNK1/2
  • LIMD1
  • mitotic phosphorylation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

LIMD1 phosphorylation in mitosis is required for mitotic progression and its tumor-suppressing activity. / Zhou, Jiuli; Zhang, Lin; Zhou, Wei; Chen, Yuanhong; Cheng, Yufeng; Dong, Jixin.

In: FEBS Journal, Vol. 286, No. 5, 01.03.2019, p. 963-974.

Research output: Contribution to journalArticle

Zhou, Jiuli ; Zhang, Lin ; Zhou, Wei ; Chen, Yuanhong ; Cheng, Yufeng ; Dong, Jixin. / LIMD1 phosphorylation in mitosis is required for mitotic progression and its tumor-suppressing activity. In: FEBS Journal. 2019 ; Vol. 286, No. 5. pp. 963-974.
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