Light-Induced Translocation of RGS9-1 and Gβ5L in Mouse Rod Photoreceptors

Mei Tian, Marisa L Zallocchi, Weimin Wang, Ching Kang Chen, Krzysztof Palczewski, Duane Delimont, Dominic E Cosgrove, You Wei Peng

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The transducin GTPase-accelerating protein complex, which determines the photoresponse duration of photoreceptors, is composed of RGS9-1, Gβ5L and R9AP. Here we report that RGS9-1 and Gβ5L change their distribution in rods during light/dark adaptation. Upon prolonged dark adaptation, RGS9-1 and Gβ5L are primarily located in rod inner segments. But very dim-light exposure quickly translocates them to the outer segments. In contrast, their anchor protein R9AP remains in the outer segment at all times. In the dark, Gβ5L's interaction with R9AP decreases significantly and RGS9-1 is phosphorylated at S475 to a significant degree. Dim light exposure leads to quick de-phosphorylation of RGS9-1. Furthermore, after prolonged dark adaptation, RGS9-1 and transducin Gα are located in different cellular compartments. These results suggest a previously unappreciated mechanism by which prolonged dark adaptation leads to increased light sensitivity in rods by dissociating RGS9-1 from R9AP and redistributing it to rod inner segments.

Original languageEnglish (US)
Article numbere58832
JournalPloS one
Volume8
Issue number3
DOIs
StatePublished - Mar 28 2013

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Dark Adaptation
rods (retina)
dark adaptation
Retinal Rod Photoreceptor Cells
Retinal Photoreceptor Cell Inner Segment
Light
mice
Transducin
Phosphorylation
Photosensitivity
GTPase-activating proteins
Ocular Adaptation
Anchors
Photophobia
dephosphorylation
Proteins
photoreceptors
duration
proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Tian, M., Zallocchi, M. L., Wang, W., Chen, C. K., Palczewski, K., Delimont, D., ... Peng, Y. W. (2013). Light-Induced Translocation of RGS9-1 and Gβ5L in Mouse Rod Photoreceptors. PloS one, 8(3), [e58832]. https://doi.org/10.1371/journal.pone.0058832

Light-Induced Translocation of RGS9-1 and Gβ5L in Mouse Rod Photoreceptors. / Tian, Mei; Zallocchi, Marisa L; Wang, Weimin; Chen, Ching Kang; Palczewski, Krzysztof; Delimont, Duane; Cosgrove, Dominic E; Peng, You Wei.

In: PloS one, Vol. 8, No. 3, e58832, 28.03.2013.

Research output: Contribution to journalArticle

Tian, Mei ; Zallocchi, Marisa L ; Wang, Weimin ; Chen, Ching Kang ; Palczewski, Krzysztof ; Delimont, Duane ; Cosgrove, Dominic E ; Peng, You Wei. / Light-Induced Translocation of RGS9-1 and Gβ5L in Mouse Rod Photoreceptors. In: PloS one. 2013 ; Vol. 8, No. 3.
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