Light and scanning electron microscopy of exfoliated bladder epithelial cells in rats fed N-[4-(5-Nitro-2-furyl)-2-thiazolyl]formamide

J. B. Jacobs, M. Arai, Samuel Monroe Cohen, G. H. Friedell

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Urine specimens obtained periodically from weanling male F344 rats fed 0.2% N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide (FANFT) for 25 weeks followed by control diet for an additional 35 weeks, were examined by light microscopy (LM) and scanning electron microscopy (SEM). LM revealed red blood cells and atypical transitional cells in the urine at 25 and 43 weeks, when microinvasive transitional cell carcinoma was present. Malignant transitional cells were seen in the urine by LM at 60 weeks, when muscle invasion was present in histologic sections. However, SEM showed pleomorphic microvilli on exfoliated urothelial cells as early as 10 weeks after the beginning of FANFT administration, and the microvilli became increasingly pleomorphic as the bladder lesions progressed to muscle invasion at 60 weeks. Noninvasive tumors were present in histologic sections of rat bladders examined at the end of 10 weeks of FANFT administration. The numerous microvilli on exfoliated bladder tumor cells were in sharp contrast to the peaked micro ridges of normal exfoliated superficial cells.

Original languageEnglish (US)
Pages (from-to)63-66
Number of pages4
JournalJournal of the National Cancer Institute
Volume57
Issue number1
DOIs
StatePublished - Jan 1 1976

Fingerprint

FANFT
Electron Scanning Microscopy
Urinary Bladder
Epithelial Cells
Light
Microvilli
Microscopy
Urine
Muscles
Transitional Cell Carcinoma
Inbred F344 Rats
Urinary Bladder Neoplasms
Erythrocytes
formamide
Diet
Neoplasms

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Light and scanning electron microscopy of exfoliated bladder epithelial cells in rats fed N-[4-(5-Nitro-2-furyl)-2-thiazolyl]formamide. / Jacobs, J. B.; Arai, M.; Cohen, Samuel Monroe; Friedell, G. H.

In: Journal of the National Cancer Institute, Vol. 57, No. 1, 01.01.1976, p. 63-66.

Research output: Contribution to journalArticle

@article{20acb7db0127493ab23a9393b8358b15,
title = "Light and scanning electron microscopy of exfoliated bladder epithelial cells in rats fed N-[4-(5-Nitro-2-furyl)-2-thiazolyl]formamide",
abstract = "Urine specimens obtained periodically from weanling male F344 rats fed 0.2{\%} N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide (FANFT) for 25 weeks followed by control diet for an additional 35 weeks, were examined by light microscopy (LM) and scanning electron microscopy (SEM). LM revealed red blood cells and atypical transitional cells in the urine at 25 and 43 weeks, when microinvasive transitional cell carcinoma was present. Malignant transitional cells were seen in the urine by LM at 60 weeks, when muscle invasion was present in histologic sections. However, SEM showed pleomorphic microvilli on exfoliated urothelial cells as early as 10 weeks after the beginning of FANFT administration, and the microvilli became increasingly pleomorphic as the bladder lesions progressed to muscle invasion at 60 weeks. Noninvasive tumors were present in histologic sections of rat bladders examined at the end of 10 weeks of FANFT administration. The numerous microvilli on exfoliated bladder tumor cells were in sharp contrast to the peaked micro ridges of normal exfoliated superficial cells.",
author = "Jacobs, {J. B.} and M. Arai and Cohen, {Samuel Monroe} and Friedell, {G. H.}",
year = "1976",
month = "1",
day = "1",
doi = "10.1093/jnci/57.1.63",
language = "English (US)",
volume = "57",
pages = "63--66",
journal = "Journal of the National Cancer Institute",
issn = "0027-8874",
publisher = "Oxford University Press",
number = "1",

}

TY - JOUR

T1 - Light and scanning electron microscopy of exfoliated bladder epithelial cells in rats fed N-[4-(5-Nitro-2-furyl)-2-thiazolyl]formamide

AU - Jacobs, J. B.

AU - Arai, M.

AU - Cohen, Samuel Monroe

AU - Friedell, G. H.

PY - 1976/1/1

Y1 - 1976/1/1

N2 - Urine specimens obtained periodically from weanling male F344 rats fed 0.2% N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide (FANFT) for 25 weeks followed by control diet for an additional 35 weeks, were examined by light microscopy (LM) and scanning electron microscopy (SEM). LM revealed red blood cells and atypical transitional cells in the urine at 25 and 43 weeks, when microinvasive transitional cell carcinoma was present. Malignant transitional cells were seen in the urine by LM at 60 weeks, when muscle invasion was present in histologic sections. However, SEM showed pleomorphic microvilli on exfoliated urothelial cells as early as 10 weeks after the beginning of FANFT administration, and the microvilli became increasingly pleomorphic as the bladder lesions progressed to muscle invasion at 60 weeks. Noninvasive tumors were present in histologic sections of rat bladders examined at the end of 10 weeks of FANFT administration. The numerous microvilli on exfoliated bladder tumor cells were in sharp contrast to the peaked micro ridges of normal exfoliated superficial cells.

AB - Urine specimens obtained periodically from weanling male F344 rats fed 0.2% N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide (FANFT) for 25 weeks followed by control diet for an additional 35 weeks, were examined by light microscopy (LM) and scanning electron microscopy (SEM). LM revealed red blood cells and atypical transitional cells in the urine at 25 and 43 weeks, when microinvasive transitional cell carcinoma was present. Malignant transitional cells were seen in the urine by LM at 60 weeks, when muscle invasion was present in histologic sections. However, SEM showed pleomorphic microvilli on exfoliated urothelial cells as early as 10 weeks after the beginning of FANFT administration, and the microvilli became increasingly pleomorphic as the bladder lesions progressed to muscle invasion at 60 weeks. Noninvasive tumors were present in histologic sections of rat bladders examined at the end of 10 weeks of FANFT administration. The numerous microvilli on exfoliated bladder tumor cells were in sharp contrast to the peaked micro ridges of normal exfoliated superficial cells.

UR - http://www.scopus.com/inward/record.url?scp=0017116915&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017116915&partnerID=8YFLogxK

U2 - 10.1093/jnci/57.1.63

DO - 10.1093/jnci/57.1.63

M3 - Article

VL - 57

SP - 63

EP - 66

JO - Journal of the National Cancer Institute

JF - Journal of the National Cancer Institute

SN - 0027-8874

IS - 1

ER -