LH-Independent testosterone secretion is mediated by the interaction between GNRH2 and its receptor within porcine testes

Amy T. Desaulniers, Rebecca A. Cederberg, Ginger A. Mills, J. Joe Ford, Clay A. Lents, Brett R. White

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Unlike classic gonadotropin-releasing hormone 1 (GNRH1), the second mammalian isoform (GNRH2) is an ineffective stimulant of gonadotropin release. Species that produce GNRH2 may not maintain a functional GNRH2 receptor (GNRHR2) due to coding errors. A full-length GNRHR2 gene has been identified in swine, but its role in reproduction requires further elucidation. Our objective was to examine the role of GNRH2 and GNRHR2 in testicular function of boars. We discovered that GNRH2 levels were higher in the testis than in the anterior pituitary gland or hypothalamus, corresponding to greater GNRHR2 abundance in the testis versus the anterior pituitary gland. Moreover, GNRH2 immunostaining was most prevalent within seminiferous tubules, whereas GNRHR2 was detected in high abundance on Leydig cells. GNRH2 pretreatment of testis explant cultures elicited testosterone secretion similar to that of human chorionic gonadotropin stimulation. Treatment of mature boars with GNRH2 elevated testosterone levels similar to those of GNRH1-treated males, despite minimal GNRH2- induced release of luteinizing hormone (LH). When pretreated with a GNRHR1 antagonist (SB-75), subsequent GNRH2 treatment stimulated low levels of testosterone secretion despite a pattern of LH release similar to that in the previous trial, suggesting that SB-75 inhibited testicular GNRHR2s. Given that pigs lack testicular GNRHR1, these data may indicate that GNRH2 and its receptor are involved in autocrine or paracrine regulation of testosterone secretion. Notably, our data are the first to suggest a biological function of a novel GNRH2-GNRHR2 system in the testes of swine.

Original languageEnglish (US)
Article number45
JournalBiology of reproduction
Volume93
Issue number2
DOIs
StatePublished - Aug 1 2015

Fingerprint

Luteinizing Hormone
Testosterone
Testis
Swine
Anterior Pituitary Gland
Gonadotropin-Releasing Hormone
Seminiferous Tubules
Leydig Cells
Chorionic Gonadotropin
Gonadotropins
Hypothalamus
Reproduction
Protein Isoforms
Genes
cetrorelix

Keywords

  • GNRH2
  • GNRHR2
  • Porcine
  • Testis
  • Testosterone

ASJC Scopus subject areas

  • Cell Biology

Cite this

LH-Independent testosterone secretion is mediated by the interaction between GNRH2 and its receptor within porcine testes. / Desaulniers, Amy T.; Cederberg, Rebecca A.; Mills, Ginger A.; Ford, J. Joe; Lents, Clay A.; White, Brett R.

In: Biology of reproduction, Vol. 93, No. 2, 45, 01.08.2015.

Research output: Contribution to journalArticle

Desaulniers, Amy T. ; Cederberg, Rebecca A. ; Mills, Ginger A. ; Ford, J. Joe ; Lents, Clay A. ; White, Brett R. / LH-Independent testosterone secretion is mediated by the interaction between GNRH2 and its receptor within porcine testes. In: Biology of reproduction. 2015 ; Vol. 93, No. 2.
@article{cc18a20e2f5443c3ba20574f540ed963,
title = "LH-Independent testosterone secretion is mediated by the interaction between GNRH2 and its receptor within porcine testes",
abstract = "Unlike classic gonadotropin-releasing hormone 1 (GNRH1), the second mammalian isoform (GNRH2) is an ineffective stimulant of gonadotropin release. Species that produce GNRH2 may not maintain a functional GNRH2 receptor (GNRHR2) due to coding errors. A full-length GNRHR2 gene has been identified in swine, but its role in reproduction requires further elucidation. Our objective was to examine the role of GNRH2 and GNRHR2 in testicular function of boars. We discovered that GNRH2 levels were higher in the testis than in the anterior pituitary gland or hypothalamus, corresponding to greater GNRHR2 abundance in the testis versus the anterior pituitary gland. Moreover, GNRH2 immunostaining was most prevalent within seminiferous tubules, whereas GNRHR2 was detected in high abundance on Leydig cells. GNRH2 pretreatment of testis explant cultures elicited testosterone secretion similar to that of human chorionic gonadotropin stimulation. Treatment of mature boars with GNRH2 elevated testosterone levels similar to those of GNRH1-treated males, despite minimal GNRH2- induced release of luteinizing hormone (LH). When pretreated with a GNRHR1 antagonist (SB-75), subsequent GNRH2 treatment stimulated low levels of testosterone secretion despite a pattern of LH release similar to that in the previous trial, suggesting that SB-75 inhibited testicular GNRHR2s. Given that pigs lack testicular GNRHR1, these data may indicate that GNRH2 and its receptor are involved in autocrine or paracrine regulation of testosterone secretion. Notably, our data are the first to suggest a biological function of a novel GNRH2-GNRHR2 system in the testes of swine.",
keywords = "GNRH2, GNRHR2, Porcine, Testis, Testosterone",
author = "Desaulniers, {Amy T.} and Cederberg, {Rebecca A.} and Mills, {Ginger A.} and Ford, {J. Joe} and Lents, {Clay A.} and White, {Brett R.}",
year = "2015",
month = "8",
day = "1",
doi = "10.1095/biolreprod.115.128082",
language = "English (US)",
volume = "93",
journal = "Biology of Reproduction",
issn = "0006-3363",
publisher = "Society for the Study of Reproduction",
number = "2",

}

TY - JOUR

T1 - LH-Independent testosterone secretion is mediated by the interaction between GNRH2 and its receptor within porcine testes

AU - Desaulniers, Amy T.

AU - Cederberg, Rebecca A.

AU - Mills, Ginger A.

AU - Ford, J. Joe

AU - Lents, Clay A.

AU - White, Brett R.

PY - 2015/8/1

Y1 - 2015/8/1

N2 - Unlike classic gonadotropin-releasing hormone 1 (GNRH1), the second mammalian isoform (GNRH2) is an ineffective stimulant of gonadotropin release. Species that produce GNRH2 may not maintain a functional GNRH2 receptor (GNRHR2) due to coding errors. A full-length GNRHR2 gene has been identified in swine, but its role in reproduction requires further elucidation. Our objective was to examine the role of GNRH2 and GNRHR2 in testicular function of boars. We discovered that GNRH2 levels were higher in the testis than in the anterior pituitary gland or hypothalamus, corresponding to greater GNRHR2 abundance in the testis versus the anterior pituitary gland. Moreover, GNRH2 immunostaining was most prevalent within seminiferous tubules, whereas GNRHR2 was detected in high abundance on Leydig cells. GNRH2 pretreatment of testis explant cultures elicited testosterone secretion similar to that of human chorionic gonadotropin stimulation. Treatment of mature boars with GNRH2 elevated testosterone levels similar to those of GNRH1-treated males, despite minimal GNRH2- induced release of luteinizing hormone (LH). When pretreated with a GNRHR1 antagonist (SB-75), subsequent GNRH2 treatment stimulated low levels of testosterone secretion despite a pattern of LH release similar to that in the previous trial, suggesting that SB-75 inhibited testicular GNRHR2s. Given that pigs lack testicular GNRHR1, these data may indicate that GNRH2 and its receptor are involved in autocrine or paracrine regulation of testosterone secretion. Notably, our data are the first to suggest a biological function of a novel GNRH2-GNRHR2 system in the testes of swine.

AB - Unlike classic gonadotropin-releasing hormone 1 (GNRH1), the second mammalian isoform (GNRH2) is an ineffective stimulant of gonadotropin release. Species that produce GNRH2 may not maintain a functional GNRH2 receptor (GNRHR2) due to coding errors. A full-length GNRHR2 gene has been identified in swine, but its role in reproduction requires further elucidation. Our objective was to examine the role of GNRH2 and GNRHR2 in testicular function of boars. We discovered that GNRH2 levels were higher in the testis than in the anterior pituitary gland or hypothalamus, corresponding to greater GNRHR2 abundance in the testis versus the anterior pituitary gland. Moreover, GNRH2 immunostaining was most prevalent within seminiferous tubules, whereas GNRHR2 was detected in high abundance on Leydig cells. GNRH2 pretreatment of testis explant cultures elicited testosterone secretion similar to that of human chorionic gonadotropin stimulation. Treatment of mature boars with GNRH2 elevated testosterone levels similar to those of GNRH1-treated males, despite minimal GNRH2- induced release of luteinizing hormone (LH). When pretreated with a GNRHR1 antagonist (SB-75), subsequent GNRH2 treatment stimulated low levels of testosterone secretion despite a pattern of LH release similar to that in the previous trial, suggesting that SB-75 inhibited testicular GNRHR2s. Given that pigs lack testicular GNRHR1, these data may indicate that GNRH2 and its receptor are involved in autocrine or paracrine regulation of testosterone secretion. Notably, our data are the first to suggest a biological function of a novel GNRH2-GNRHR2 system in the testes of swine.

KW - GNRH2

KW - GNRHR2

KW - Porcine

KW - Testis

KW - Testosterone

UR - http://www.scopus.com/inward/record.url?scp=84941561536&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84941561536&partnerID=8YFLogxK

U2 - 10.1095/biolreprod.115.128082

DO - 10.1095/biolreprod.115.128082

M3 - Article

C2 - 26134865

AN - SCOPUS:84941561536

VL - 93

JO - Biology of Reproduction

JF - Biology of Reproduction

SN - 0006-3363

IS - 2

M1 - 45

ER -