Lens epithelium-derived growth factor: Neuroprotection on rat retinal damage induced by N-methyl-D-aspartate

Yasuya Inomata, Akira Hirata, Takahisa Koga, Akira Kimura, Dhirendra P. Singh, Toshimichi Shinohara, Hidenobu Tanihara

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The purpose of this study is to investigate possible neuroprotective effects of lens epithelium-derived growth factor (LEDGF) against cell death induced by N-methyl-D-aspartate (NMDA) in the rat retina. LEDGF and/or NMDA were intravitreally injected into rat eyes. NMDA-induced retinal death and protective effects of LEDGF were evaluated by morphometric analysis, cell numbers in the ganglion cell layer (GCL) and the thickness of the inner plexiform layer (IPL). Retrograde labeling with a fluorescent tracer (Fluoro-Gold) was applied for counting retinal ganglion cells (RGCs) that survived after NMDA injection. Terminal deoxyribonucleotidyl transferase (TdT)-mediated fluroscein-16-dUTP nick end-labeling (TUNEL) staining was used to evaluate of retinal cell death. Morphometric analysis and retrograde labeling analysis showed that retinal damage induced by NMDA was protected significantly by LEDGF. TUNEL assay revealed that pretreatment with LEDGF prevents NMDA-induced apoptosis. Retinal damage (ganglion and amacrine cells) induced by NMDA was protected by an intravitreal injection of LEDGF.

Original languageEnglish (US)
Pages (from-to)163-170
Number of pages8
JournalBrain Research
Volume991
Issue number1-2
DOIs
StatePublished - Nov 21 2003

Fingerprint

N-Methylaspartate
Retinal Ganglion Cells
In Situ Nick-End Labeling
Cell Death
Amacrine Cells
Intravitreal Injections
DNA Nucleotidylexotransferase
Neuroprotective Agents
lens epithelium-derived growth factor
Neuroprotection
Ganglia
Retina
Cell Count
Apoptosis
Staining and Labeling
Injections

Keywords

  • Lens epithelium-derived growth factor
  • N-methyl-D-aspartate
  • Neuroprotection
  • Rat retina
  • Retinal ganglion cell

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Lens epithelium-derived growth factor : Neuroprotection on rat retinal damage induced by N-methyl-D-aspartate. / Inomata, Yasuya; Hirata, Akira; Koga, Takahisa; Kimura, Akira; Singh, Dhirendra P.; Shinohara, Toshimichi; Tanihara, Hidenobu.

In: Brain Research, Vol. 991, No. 1-2, 21.11.2003, p. 163-170.

Research output: Contribution to journalArticle

Inomata, Yasuya ; Hirata, Akira ; Koga, Takahisa ; Kimura, Akira ; Singh, Dhirendra P. ; Shinohara, Toshimichi ; Tanihara, Hidenobu. / Lens epithelium-derived growth factor : Neuroprotection on rat retinal damage induced by N-methyl-D-aspartate. In: Brain Research. 2003 ; Vol. 991, No. 1-2. pp. 163-170.
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