Lens epithelium-derived growth factor

Increased resistance to thermal and oxidative stresses

Dhirendra P Singh, Nobuyuki Ohguro, Leo T. Chylack, Toshimichi Shinohara

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

PURPOSE. To investigate the role of lens epithelium- derived growth factor (LEDGF) in lens epithelial cells subjected to heat or oxidative stress and to determine the localization of LEDGF in lens epithelial cells at different temperatures. METHODS. Mouse lens epithelial cells overexpressing a fusion protein between a green fluorescent protein (GFP) and LEDGF (GFP- LEDGF) were cultured for up to 7 days at various temperatures or for 24 hours in the presence of hydrogen peroxide. Translocation of GFP-LEDGF in the lens epithelial cells was monitored with a fluorescence microscope. Cell survival was determined with the trypan blue dye exclusion test. Expression of heat shock proteins (Hsps) was studied with protein blot analyses using antibody probes. RESULTS. LEDGF was found in the cytosol of lens epithelial cells at 4°C, 15°C, and 28°C, and in the nucleus and nucleolus at 37°C. At 41 °C, it was apparent at higher levels in the cytosol, nucleus, and nucleolus. Lens epithelial cells overexpressing LEDGF manifested resistance to thermal and oxidative stress. Levels of Hsp 27, and αB-crystallin were elevated in these cells. Depriving lens epithelial cells of LEDGF with anti-LEDGF antibodies reduced nuclear localization of LEDGF and induced cell death. CONCLUSIONS. The overexpression of LEDGF in lens epithelial cells conferred resistance to thermal and oxidative stress. The mechanism of this resistance appears to involve the increased expression of Hsp 27 and αB-crystallin.

Original languageEnglish (US)
Pages (from-to)1444-1451
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume40
Issue number7
StatePublished - Jun 9 1999

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Oxidative Stress
Hot Temperature
Lenses
Epithelial Cells
Crystallins
Green Fluorescent Proteins
Cytosol
lens epithelium-derived growth factor
Temperature
Trypan Blue
Antibodies
Heat-Shock Proteins
Hydrogen Peroxide
Cell Survival
Proteins
Cell Death
Coloring Agents
Fluorescence

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Lens epithelium-derived growth factor : Increased resistance to thermal and oxidative stresses. / Singh, Dhirendra P; Ohguro, Nobuyuki; Chylack, Leo T.; Shinohara, Toshimichi.

In: Investigative Ophthalmology and Visual Science, Vol. 40, No. 7, 09.06.1999, p. 1444-1451.

Research output: Contribution to journalArticle

Singh, Dhirendra P ; Ohguro, Nobuyuki ; Chylack, Leo T. ; Shinohara, Toshimichi. / Lens epithelium-derived growth factor : Increased resistance to thermal and oxidative stresses. In: Investigative Ophthalmology and Visual Science. 1999 ; Vol. 40, No. 7. pp. 1444-1451.
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