LEDGF: Survival of embryonic chick retinal photoreceptor cells

Makoto Nakamura, Dhirendra P. Singh, Eri Kubo, Leo T. Chylack, Toshimichi Shinohara

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

PURPOSE. Lens epithelium-derived growth factor (LEDGF) is a novel adhesive, survival, and growth factor for lens epithelial cells, keratinocytes, fibroblasts, and cos7 cells. In the presence of LEDGF, these cells acquire resistance to environmental stresses, and in the absence of LEDGF they die. The effects of LEDGF on survival of embryonic chick retinal photoreceptor cells under serum starvation and heat stress were studied. METHODS. The expression pattern of LEDGF in embryonic chick retinal photoreceptor cells was investigated with protein blot analysis and immunohistochemistry using antibodies (Abs) to LEDGF. Retinal cells were cultured in serum-free medium for up to 6 days in the presence of varying amounts of LEDGF at 37°or 41°C. The photoreceptor cells were immunostained with Abs to arrestin and counted to evaluate the photoreceptor cell viability. Heat shock proteins in the cultured cells were quantified by protein blot analysis with Ab probes and semiquantitative reverse transcription-polymerase chain reaction analysis. RESULTS. LEDGF was found predominantly in the nucleus of neuroretinal cells, including photoreceptor cells. In the presence of LEDGF, photoreceptor cells manifested increased resistance to serum starvation and thermal stress and survived for a longer period. The levels of heat shock protein 90 were elevated in those cells. Most retinal cells died in the absence of LEDGF. CONCLUSIONS. LEDGF enhanced survival of retinal photoreceptor cells under serum starvation and heat stress. Thus, LEDGF has a potency to enhance survival of neuronal cell types against environmental stresses, and it may be applicable as a therapeutic agent for those cells.

Original languageEnglish (US)
Pages (from-to)1168-1175
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume41
Issue number5
StatePublished - Apr 15 2000

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Vertebrate Photoreceptor Cells
Photoreceptor Cells
Starvation
Hot Temperature
lens epithelium-derived growth factor
Cultured Cells
Cell Survival
Serum
Arrestin
HSP90 Heat-Shock Proteins
Antibodies
Serum-Free Culture Media
Heat-Shock Proteins
Cell Nucleus
Keratinocytes
Adhesives
Lenses
Reverse Transcription
Intercellular Signaling Peptides and Proteins
Proteins

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Nakamura, M., Singh, D. P., Kubo, E., Chylack, L. T., & Shinohara, T. (2000). LEDGF: Survival of embryonic chick retinal photoreceptor cells. Investigative Ophthalmology and Visual Science, 41(5), 1168-1175.

LEDGF : Survival of embryonic chick retinal photoreceptor cells. / Nakamura, Makoto; Singh, Dhirendra P.; Kubo, Eri; Chylack, Leo T.; Shinohara, Toshimichi.

In: Investigative Ophthalmology and Visual Science, Vol. 41, No. 5, 15.04.2000, p. 1168-1175.

Research output: Contribution to journalArticle

Nakamura, M, Singh, DP, Kubo, E, Chylack, LT & Shinohara, T 2000, 'LEDGF: Survival of embryonic chick retinal photoreceptor cells', Investigative Ophthalmology and Visual Science, vol. 41, no. 5, pp. 1168-1175.
Nakamura, Makoto ; Singh, Dhirendra P. ; Kubo, Eri ; Chylack, Leo T. ; Shinohara, Toshimichi. / LEDGF : Survival of embryonic chick retinal photoreceptor cells. In: Investigative Ophthalmology and Visual Science. 2000 ; Vol. 41, No. 5. pp. 1168-1175.
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