Lateral mobility of presynaptic L-Type calcium channels at photoreceptor ribbon synapses

Aaron J. Mercer, Minghui Chen, Wallace B Thoreson

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

At most synapses, presynaptic Ca2+ channels are positioned near vesicle release sites, and increasing this distance reduces synaptic strength. We examined the lateral membrane mobility of presynaptic L-type Ca2+ channels at photoreceptor ribbon synapses of the tiger salamander (Ambystoma tigrinum) retina. Movements of individual Ca2+ channels were tracked by coupling quantum dots to an antibody against the extracellular α2δ4 Ca2+ channel subunit. α2δ4 antibodies labeled photoreceptor terminals and colocalized with antibodies to synaptic vesicle glycoprotein 2 and voltage-gated Ca2+ channel 1.4 (CaV1.4) α1 subunits. The results show that Ca2+ channels are dynamic and move within a confined region beneath the synaptic ribbon. The size of this confinement area is regulated by actin and membrane cholesterol. Fusion of nearby synaptic vesicles caused jumps in Ca2+ channel position, propelling them toward the outer edge of the confinement domain. Channels rebounded rapidly toward the center. Thus, although CaV channels are mobile, molecular scaffolds confine them beneath the ribbon to maintain neurotransmission even at high release rates.

Original languageEnglish (US)
Pages (from-to)4397-4406
Number of pages10
JournalJournal of Neuroscience
Volume31
Issue number12
DOIs
StatePublished - Mar 23 2011

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L-Type Calcium Channels
Ambystoma
Synapses
Synaptic Vesicles
Antibodies
Quantum Dots
Membranes
Synaptic Transmission
Retina
Actins
Glycoproteins
Cholesterol

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine(all)

Cite this

Lateral mobility of presynaptic L-Type calcium channels at photoreceptor ribbon synapses. / Mercer, Aaron J.; Chen, Minghui; Thoreson, Wallace B.

In: Journal of Neuroscience, Vol. 31, No. 12, 23.03.2011, p. 4397-4406.

Research output: Contribution to journalArticle

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AB - At most synapses, presynaptic Ca2+ channels are positioned near vesicle release sites, and increasing this distance reduces synaptic strength. We examined the lateral membrane mobility of presynaptic L-type Ca2+ channels at photoreceptor ribbon synapses of the tiger salamander (Ambystoma tigrinum) retina. Movements of individual Ca2+ channels were tracked by coupling quantum dots to an antibody against the extracellular α2δ4 Ca2+ channel subunit. α2δ4 antibodies labeled photoreceptor terminals and colocalized with antibodies to synaptic vesicle glycoprotein 2 and voltage-gated Ca2+ channel 1.4 (CaV1.4) α1 subunits. The results show that Ca2+ channels are dynamic and move within a confined region beneath the synaptic ribbon. The size of this confinement area is regulated by actin and membrane cholesterol. Fusion of nearby synaptic vesicles caused jumps in Ca2+ channel position, propelling them toward the outer edge of the confinement domain. Channels rebounded rapidly toward the center. Thus, although CaV channels are mobile, molecular scaffolds confine them beneath the ribbon to maintain neurotransmission even at high release rates.

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