Abstract
Physical characterization of the mitochondrial genome derived from the obligate mosquito parasite, Romanomermis culicivorax has generated some surprising physical properties regarding the molecular structure of nematode mitochondrial DNA (mtDNA). Restriction enzyme analysis of this mtDNA has revealed a mitochondrial genome size of approximately 26 kb, the largest metazoan mtDNA reported to date. Isofemale lineages are monomorphic for one of three size variants, differing by 500-1,000 base pairs, present in our original field population. Cloned hybridization probes derived from a single region exhibiting a 600 by size polymorphism share strong homology with several spatially separated sites distributed about the mtDNA. This suggests that the homology is a result of repeated DNA sequence elements contained within this mitochondrial genome that contribute to mtDNA size polymorphism.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 71-77 |
| Number of pages | 7 |
| Journal | Current Genetics |
| Volume | 11 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 1 1986 |
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Keywords
- Mitochondrial DNA
- Molecular evolution
- Population heterogeneity
- Recombinant DNA
ASJC Scopus subject areas
- Genetics
Cite this
Large mitochondrial genome and mitochondrial DNA size polymorphism in the mosquito parasite, Romanomermis culicivorax. / Powers, Thomas O.; Plazzer, E. G.; Hyman, Bradley C.
In: Current Genetics, Vol. 11, No. 1, 01.10.1986, p. 71-77.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Large mitochondrial genome and mitochondrial DNA size polymorphism in the mosquito parasite, Romanomermis culicivorax
AU - Powers, Thomas O.
AU - Plazzer, E. G.
AU - Hyman, Bradley C.
PY - 1986/10/1
Y1 - 1986/10/1
N2 - Physical characterization of the mitochondrial genome derived from the obligate mosquito parasite, Romanomermis culicivorax has generated some surprising physical properties regarding the molecular structure of nematode mitochondrial DNA (mtDNA). Restriction enzyme analysis of this mtDNA has revealed a mitochondrial genome size of approximately 26 kb, the largest metazoan mtDNA reported to date. Isofemale lineages are monomorphic for one of three size variants, differing by 500-1,000 base pairs, present in our original field population. Cloned hybridization probes derived from a single region exhibiting a 600 by size polymorphism share strong homology with several spatially separated sites distributed about the mtDNA. This suggests that the homology is a result of repeated DNA sequence elements contained within this mitochondrial genome that contribute to mtDNA size polymorphism.
AB - Physical characterization of the mitochondrial genome derived from the obligate mosquito parasite, Romanomermis culicivorax has generated some surprising physical properties regarding the molecular structure of nematode mitochondrial DNA (mtDNA). Restriction enzyme analysis of this mtDNA has revealed a mitochondrial genome size of approximately 26 kb, the largest metazoan mtDNA reported to date. Isofemale lineages are monomorphic for one of three size variants, differing by 500-1,000 base pairs, present in our original field population. Cloned hybridization probes derived from a single region exhibiting a 600 by size polymorphism share strong homology with several spatially separated sites distributed about the mtDNA. This suggests that the homology is a result of repeated DNA sequence elements contained within this mitochondrial genome that contribute to mtDNA size polymorphism.
KW - Mitochondrial DNA
KW - Molecular evolution
KW - Population heterogeneity
KW - Recombinant DNA
UR - http://www.scopus.com/inward/record.url?scp=0022847444&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022847444&partnerID=8YFLogxK
U2 - 10.1007/BF00389428
DO - 10.1007/BF00389428
M3 - Article
C2 - 2834082
AN - SCOPUS:0022847444
VL - 11
SP - 71
EP - 77
JO - Current Genetics
JF - Current Genetics
SN - 0172-8083
IS - 1
ER -