Large granular lymphocytes from SCID horses develop potent cytotoxic activity after treatment with human recombinant interleukin 2

N. S. Magnuson, L. E. Perryman, C. R. Wyatt, P. H. Mason, James E Talmadge

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Abstract

Peripheral blood mononuclear cells from foals with hereditary severe combined immunodeficiency (SCID) have morphologic characteristics of large granular lymphocytes (LGL). Attemps to demonstrate cytotoxic activity were without success unless the LGL were incubated with 100 U of human recombinant interleukin 2 (rIL 2)/ml for 24 hr. With rIL 2 incubation, low effector to target ratios (10:1) consistently yielded high levels of cytotoxic activity (30 to 50%) in a standard 4-hr 51Cr-release assay using YAC-1 lymphoma of K562 erythroleukemia cell lines as targets. Monoclonal antibody EqT12 reacted with a large percentage of these cells, and the level of cytotoxic activity was directly correlated to the percentage of EqT12+ cells in the preparation. In normal horses, the percentage of circulating EqT12+ cells is low (5%), while at the same time, cytotoxic activity is not usually detectable even with rIL 2 incubation. In contrast, the percentage of EqT12+ cells in blood of SCID horses is high (up to 40%), as is the IL 2-inducible cytotoxic activity. These results indicate that cytotoxic cells with morphologic and functional characteristics of natural killer cells are produced by horses with SCID.

Original languageEnglish (US)
Pages (from-to)61-67
Number of pages7
JournalJournal of Immunology
Volume139
Issue number1
StatePublished - Aug 21 1987

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Severe Combined Immunodeficiency
Horses
Interleukin-2
Lymphocytes
Blood Cells
Leukemia, Erythroblastic, Acute
K562 Cells
Natural Killer Cells
Lymphoma
Monoclonal Antibodies
Cell Line

ASJC Scopus subject areas

  • Immunology

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Large granular lymphocytes from SCID horses develop potent cytotoxic activity after treatment with human recombinant interleukin 2. / Magnuson, N. S.; Perryman, L. E.; Wyatt, C. R.; Mason, P. H.; Talmadge, James E.

In: Journal of Immunology, Vol. 139, No. 1, 21.08.1987, p. 61-67.

Research output: Contribution to journalArticle

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abstract = "Peripheral blood mononuclear cells from foals with hereditary severe combined immunodeficiency (SCID) have morphologic characteristics of large granular lymphocytes (LGL). Attemps to demonstrate cytotoxic activity were without success unless the LGL were incubated with 100 U of human recombinant interleukin 2 (rIL 2)/ml for 24 hr. With rIL 2 incubation, low effector to target ratios (10:1) consistently yielded high levels of cytotoxic activity (30 to 50{\%}) in a standard 4-hr 51Cr-release assay using YAC-1 lymphoma of K562 erythroleukemia cell lines as targets. Monoclonal antibody EqT12 reacted with a large percentage of these cells, and the level of cytotoxic activity was directly correlated to the percentage of EqT12+ cells in the preparation. In normal horses, the percentage of circulating EqT12+ cells is low (5{\%}), while at the same time, cytotoxic activity is not usually detectable even with rIL 2 incubation. In contrast, the percentage of EqT12+ cells in blood of SCID horses is high (up to 40{\%}), as is the IL 2-inducible cytotoxic activity. These results indicate that cytotoxic cells with morphologic and functional characteristics of natural killer cells are produced by horses with SCID.",
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