Laparotomy attenuates lipopolysaccharide-induced gastric bleeding in the rat

Jeremy L. Ward, Benjamin A. Delano, Sasha D. Adams, Elizabeth E. Mercer, David W. Mercer

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Lipopolysaccharide (LPS) increases systemic inflammation and causes duodenogastric reflux of bile and gastric bleeding. Laparotomy prevents gastric injury from the luminal irritant bile, but its effects on LPS-induced gastric injury are unknown. We hypothesized that laparotomy would diminish inflammation and attenuate gastric bleeding caused by LPS. In the rat, laparotomy, done either before or after administration of LPS, attenuated LPS-induced bile reflux, gastric bleeding, and cyclooxygenase-2, but not inducible nitric oxide synthase, expression when compared to controls given LPS. Laparotomy also blunted LPS-induced changes in serum cytokine production. These data suggest that laparotomy has gastroprotective effects by preventing LPS-induced bile reflux and gastric bleeding and by a mechanism mediated, at least in part by cyclooxygenase-2.

Original languageEnglish (US)
Pages (from-to)902-910
Number of pages9
JournalDigestive Diseases and Sciences
Volume55
Issue number4
DOIs
StatePublished - Apr 1 2010

Fingerprint

Laparotomy
Lipopolysaccharides
Stomach
Hemorrhage
Bile Reflux
Cyclooxygenase 2
Bile
Duodenogastric Reflux
Inflammation
Irritants
Wounds and Injuries
Nitric Oxide Synthase Type II
Cytokines
Serum

Keywords

  • COX2
  • Duodenogastric reflux
  • Laparotomy
  • Lipopolysaccharide

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

Cite this

Laparotomy attenuates lipopolysaccharide-induced gastric bleeding in the rat. / Ward, Jeremy L.; Delano, Benjamin A.; Adams, Sasha D.; Mercer, Elizabeth E.; Mercer, David W.

In: Digestive Diseases and Sciences, Vol. 55, No. 4, 01.04.2010, p. 902-910.

Research output: Contribution to journalArticle

Ward, Jeremy L. ; Delano, Benjamin A. ; Adams, Sasha D. ; Mercer, Elizabeth E. ; Mercer, David W. / Laparotomy attenuates lipopolysaccharide-induced gastric bleeding in the rat. In: Digestive Diseases and Sciences. 2010 ; Vol. 55, No. 4. pp. 902-910.
@article{0b77528360774e99914845c117605e4f,
title = "Laparotomy attenuates lipopolysaccharide-induced gastric bleeding in the rat",
abstract = "Lipopolysaccharide (LPS) increases systemic inflammation and causes duodenogastric reflux of bile and gastric bleeding. Laparotomy prevents gastric injury from the luminal irritant bile, but its effects on LPS-induced gastric injury are unknown. We hypothesized that laparotomy would diminish inflammation and attenuate gastric bleeding caused by LPS. In the rat, laparotomy, done either before or after administration of LPS, attenuated LPS-induced bile reflux, gastric bleeding, and cyclooxygenase-2, but not inducible nitric oxide synthase, expression when compared to controls given LPS. Laparotomy also blunted LPS-induced changes in serum cytokine production. These data suggest that laparotomy has gastroprotective effects by preventing LPS-induced bile reflux and gastric bleeding and by a mechanism mediated, at least in part by cyclooxygenase-2.",
keywords = "COX2, Duodenogastric reflux, Laparotomy, Lipopolysaccharide",
author = "Ward, {Jeremy L.} and Delano, {Benjamin A.} and Adams, {Sasha D.} and Mercer, {Elizabeth E.} and Mercer, {David W.}",
year = "2010",
month = "4",
day = "1",
doi = "10.1007/s10620-009-0800-x",
language = "English (US)",
volume = "55",
pages = "902--910",
journal = "Digestive Diseases and Sciences",
issn = "0163-2116",
publisher = "Springer New York",
number = "4",

}

TY - JOUR

T1 - Laparotomy attenuates lipopolysaccharide-induced gastric bleeding in the rat

AU - Ward, Jeremy L.

AU - Delano, Benjamin A.

AU - Adams, Sasha D.

AU - Mercer, Elizabeth E.

AU - Mercer, David W.

PY - 2010/4/1

Y1 - 2010/4/1

N2 - Lipopolysaccharide (LPS) increases systemic inflammation and causes duodenogastric reflux of bile and gastric bleeding. Laparotomy prevents gastric injury from the luminal irritant bile, but its effects on LPS-induced gastric injury are unknown. We hypothesized that laparotomy would diminish inflammation and attenuate gastric bleeding caused by LPS. In the rat, laparotomy, done either before or after administration of LPS, attenuated LPS-induced bile reflux, gastric bleeding, and cyclooxygenase-2, but not inducible nitric oxide synthase, expression when compared to controls given LPS. Laparotomy also blunted LPS-induced changes in serum cytokine production. These data suggest that laparotomy has gastroprotective effects by preventing LPS-induced bile reflux and gastric bleeding and by a mechanism mediated, at least in part by cyclooxygenase-2.

AB - Lipopolysaccharide (LPS) increases systemic inflammation and causes duodenogastric reflux of bile and gastric bleeding. Laparotomy prevents gastric injury from the luminal irritant bile, but its effects on LPS-induced gastric injury are unknown. We hypothesized that laparotomy would diminish inflammation and attenuate gastric bleeding caused by LPS. In the rat, laparotomy, done either before or after administration of LPS, attenuated LPS-induced bile reflux, gastric bleeding, and cyclooxygenase-2, but not inducible nitric oxide synthase, expression when compared to controls given LPS. Laparotomy also blunted LPS-induced changes in serum cytokine production. These data suggest that laparotomy has gastroprotective effects by preventing LPS-induced bile reflux and gastric bleeding and by a mechanism mediated, at least in part by cyclooxygenase-2.

KW - COX2

KW - Duodenogastric reflux

KW - Laparotomy

KW - Lipopolysaccharide

UR - http://www.scopus.com/inward/record.url?scp=77950342223&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77950342223&partnerID=8YFLogxK

U2 - 10.1007/s10620-009-0800-x

DO - 10.1007/s10620-009-0800-x

M3 - Article

C2 - 19390968

AN - SCOPUS:77950342223

VL - 55

SP - 902

EP - 910

JO - Digestive Diseases and Sciences

JF - Digestive Diseases and Sciences

SN - 0163-2116

IS - 4

ER -