Labeling of Sulfhydryl Groups in Intact Mammalian Cells with Coumarins

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Abstract

4-(Bromomethyl)-6,7-dimethoxy-2-oxo-2H-benzopyran (1) and its 3-[125I] iodo derivative (2) were reacted with sulfhydryl groups of proteins in viable Chinese hamster V79 lung fibroblasts. Pretreatment of cells with dithiothreitol (DTT) as the reducing agent was necessary to give detectable levels of fluorescence. In the absence of DTT, V79 cells incorporated about 1 × 10-3 pCi/cell of the radioiodinated, nocarrier- added derivative; following incubation of cells with 320 μM DTT the uptake was nearly doubled (1.87 × 10-3 pCi/cell).

Original languageEnglish (US)
Pages (from-to)305-307
Number of pages3
JournalBioconjugate Chemistry
Volume4
Issue number4
DOIs
Publication statusPublished - Jul 1 1993

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ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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