Label-free characterization of exosome via surface enhanced Raman spectroscopy for the early detection of pancreatic cancer

Joseph Carmicheal, Chihiro Hayashi, Xi Huang, Lei Liu, Yao Lu, Alexey V Krasnoslobodtsev, Alexander Lushnikov, Prakash G. Kshirsagar, Asish Patel, Maneesh Jain, Yuri L Lyubchenko, Yongfeng Lu, Surinder Kumar Batra, Sukwinder Kaur

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Pancreatic cancer is a highly lethal malignancy. Lack of early diagnostic markers makes timely detection of pancreatic cancer a highly challenging endeavor. Exosomes have emerged as information-rich cancer specific biomarkers. However, characterization of tumor-specific exosomes has been challenging. This study investigated the proof of principle that exosomes could be used for the detection of pancreatic cancer. Label-free analysis of exosomes purified from normal and pancreatic cancer cell lines was performed using surface enhanced Raman Spectroscopy (SERS) and principal component differential function analysis (PC-DFA), to identify tumor-specific spectral signatures. This method differentiated exosomes originating from pancreatic cancer or normal pancreatic epithelial cell lines with 90% accuracy. The cell line trained PC-DFA algorithm was next applied to SERS spectra of serum-purified exosomes. This method exhibited up to 87% and 90% predictive accuracy for HC and EPC individual samples, respectively. Overall, our study identified utility of SERS spectral signature for deciphering exosomal surface signature.

Original languageEnglish (US)
Pages (from-to)88-96
Number of pages9
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume16
DOIs
StatePublished - Feb 1 2019

Fingerprint

Exosomes
Raman Spectrum Analysis
Pancreatic Neoplasms
Early Detection of Cancer
Raman spectroscopy
Labels
Tumors
Cells
Cell Line
Biomarkers
Neoplasms
Tumor Biomarkers
Epithelial Cells
Serum

Keywords

  • Exosome
  • Label-free
  • Liquid biopsy
  • Pancreatic cancer
  • Surface enhanced Raman spectroscopy

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

Cite this

Label-free characterization of exosome via surface enhanced Raman spectroscopy for the early detection of pancreatic cancer. / Carmicheal, Joseph; Hayashi, Chihiro; Huang, Xi; Liu, Lei; Lu, Yao; Krasnoslobodtsev, Alexey V; Lushnikov, Alexander; Kshirsagar, Prakash G.; Patel, Asish; Jain, Maneesh; Lyubchenko, Yuri L; Lu, Yongfeng; Batra, Surinder Kumar; Kaur, Sukwinder.

In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 16, 01.02.2019, p. 88-96.

Research output: Contribution to journalArticle

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AU - Lu, Yongfeng

AU - Batra, Surinder Kumar

AU - Kaur, Sukwinder

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