KLHL6 is preferentially expressed in germinal center-derived b-cell lymphomas

Christian A. Kunder, Giovanna Roncador, Ranjana H. Advani, Gabriela Gualco, Carlos E. Bacchi, Jean M. Sabile, Izidore S. Lossos, Kexin Nie, Robert John Tibshirani, Michael R. Green, Ash A. Alizadeh, Yasodha Natkunam

Research output: Contribution to journalArticle

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Abstract

Objectives: KLHL6 is a recently described BTB-Kelch protein with selective expression in lymphoid tissues and is most strongly expressed in germinal center B cells. Methods: Using gene expression profiling as well as immunohistochemistry with an anti-KLHL6 monoclonal antibody, we have characterized the expression of this molecule in normal and neoplastic tissues. Protein expression was evaluated in 1,058 hematopoietic neoplasms. Results: Consistent with its discovery as a germinal center marker, KLHL6 was positive mainly in B-cell neoplasms of germinal center derivation, including 95% of follicular lymphomas (106/112). B-cell lymphomas of non-germinal center derivation were generally negative (0/33 chronic lymphocytic leukemias/small lymphocytic lymphomas, 3/49 marginal zone lymphomas, and 2/66 mantle cell lymphomas). Conclusions: In addition to other germinal center markers, including BCL6, CD10, HGAL, and LMO2, KLHL6 immunohistochemistry may prove a useful adjunct in the diagnosis and future classification of B-cell lymphomas.

Original languageEnglish (US)
Pages (from-to)465-476
Number of pages12
JournalAmerican journal of clinical pathology
Volume148
Issue number6
DOIs
StatePublished - Dec 1 2017

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Germinal Center
Lymphoma
B-Cell Lymphoma
B-Cell Chronic Lymphocytic Leukemia
B-Lymphocytes
Immunohistochemistry
Mantle-Cell Lymphoma
Follicular Lymphoma
Gene Expression Profiling
Lymphoid Tissue
Hematologic Neoplasms
Proteins
Monoclonal Antibodies
Neoplasms

Keywords

  • Hematopathology
  • Immunohistochemistry
  • KLHL6
  • Lymphoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Kunder, C. A., Roncador, G., Advani, R. H., Gualco, G., Bacchi, C. E., Sabile, J. M., ... Natkunam, Y. (2017). KLHL6 is preferentially expressed in germinal center-derived b-cell lymphomas. American journal of clinical pathology, 148(6), 465-476. https://doi.org/10.1093/AJCP/AQX099

KLHL6 is preferentially expressed in germinal center-derived b-cell lymphomas. / Kunder, Christian A.; Roncador, Giovanna; Advani, Ranjana H.; Gualco, Gabriela; Bacchi, Carlos E.; Sabile, Jean M.; Lossos, Izidore S.; Nie, Kexin; Tibshirani, Robert John; Green, Michael R.; Alizadeh, Ash A.; Natkunam, Yasodha.

In: American journal of clinical pathology, Vol. 148, No. 6, 01.12.2017, p. 465-476.

Research output: Contribution to journalArticle

Kunder, CA, Roncador, G, Advani, RH, Gualco, G, Bacchi, CE, Sabile, JM, Lossos, IS, Nie, K, Tibshirani, RJ, Green, MR, Alizadeh, AA & Natkunam, Y 2017, 'KLHL6 is preferentially expressed in germinal center-derived b-cell lymphomas', American journal of clinical pathology, vol. 148, no. 6, pp. 465-476. https://doi.org/10.1093/AJCP/AQX099
Kunder CA, Roncador G, Advani RH, Gualco G, Bacchi CE, Sabile JM et al. KLHL6 is preferentially expressed in germinal center-derived b-cell lymphomas. American journal of clinical pathology. 2017 Dec 1;148(6):465-476. https://doi.org/10.1093/AJCP/AQX099
Kunder, Christian A. ; Roncador, Giovanna ; Advani, Ranjana H. ; Gualco, Gabriela ; Bacchi, Carlos E. ; Sabile, Jean M. ; Lossos, Izidore S. ; Nie, Kexin ; Tibshirani, Robert John ; Green, Michael R. ; Alizadeh, Ash A. ; Natkunam, Yasodha. / KLHL6 is preferentially expressed in germinal center-derived b-cell lymphomas. In: American journal of clinical pathology. 2017 ; Vol. 148, No. 6. pp. 465-476.
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abstract = "Objectives: KLHL6 is a recently described BTB-Kelch protein with selective expression in lymphoid tissues and is most strongly expressed in germinal center B cells. Methods: Using gene expression profiling as well as immunohistochemistry with an anti-KLHL6 monoclonal antibody, we have characterized the expression of this molecule in normal and neoplastic tissues. Protein expression was evaluated in 1,058 hematopoietic neoplasms. Results: Consistent with its discovery as a germinal center marker, KLHL6 was positive mainly in B-cell neoplasms of germinal center derivation, including 95{\%} of follicular lymphomas (106/112). B-cell lymphomas of non-germinal center derivation were generally negative (0/33 chronic lymphocytic leukemias/small lymphocytic lymphomas, 3/49 marginal zone lymphomas, and 2/66 mantle cell lymphomas). Conclusions: In addition to other germinal center markers, including BCL6, CD10, HGAL, and LMO2, KLHL6 immunohistochemistry may prove a useful adjunct in the diagnosis and future classification of B-cell lymphomas.",
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