Kinetic studies of biological interactions by affinity chromatography

John E. Schiel, David S Hage

Research output: Contribution to journalReview article

43 Citations (Scopus)

Abstract

The rates at which biological interactions occur can provide important information on the mechanism and behavior of such processes in living systems. This paper will discuss how affinity chromatography can be used as a tool to examine the kinetics of biological interactions. This approach, referred to here as biointeraction chromatography, uses a column with an immobilized binding agent to examine the association or dissociation of this agent with other compounds. The use of HPLC-based affinity columns in kinetic studies has received particular attention in recent years. Advantages of using HPLC with affinity chromatography for this purpose include the ability to reuse the same ligand within a column for a large number of experiments, and the good precision and accuracy of this approach. A number of techniques are available for kinetic studies through the use of affinity columns and biointeraction chromatography. These approaches include plate height measurements, peak profiling, peak fitting, split-peak measurements, and peak decay analysis. The general principles for each of these methods are discussed in this paper and some recent applications of these techniques are presented. The advantages and potential limitations of each approach are also considered.

Original languageEnglish (US)
Pages (from-to)1507-1522
Number of pages16
JournalJournal of Separation Science
Volume32
Issue number10
DOIs
StatePublished - Jul 13 2009

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Affinity chromatography
Chromatography
Kinetics
Ligands
Association reactions
Experiments

Keywords

  • Affinity chromatography
  • Biointeraction chromatography
  • Kinetics
  • Rate constants

ASJC Scopus subject areas

  • Analytical Chemistry
  • Filtration and Separation

Cite this

Kinetic studies of biological interactions by affinity chromatography. / Schiel, John E.; Hage, David S.

In: Journal of Separation Science, Vol. 32, No. 10, 13.07.2009, p. 1507-1522.

Research output: Contribution to journalReview article

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