Kinetic modeling of photoautotropic growth and neutral lipid accumulation in terms of ammonium concentration in Chlamydomonas reinhardtii

Rahul Tevatia, Yaşar Demirel, Paul Blum

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

This study focuses on the cell growth and the neutral lipid production modeling of Chlamydomonas reinhardtii in terms of different ammonium concentrations. Autotrophy was maintained during growth in a double walled bioreactor, using Tris Phosphate (TP medium) with only CO2 and NH4Cl as sole sources of carbon and nitrogen, respectively. Nitrogen depletion results in an increase in neutral lipid production with an indirect effect on the growth of algal cells. Modified Baranyi-Roberts and logistic equations were used to describe the cell growth whereas Luedeking-Piret equation was used for neutral lipid production kinetics. Sensitivity analysis shows that the model equations satisfactorily predict the cell growth and lipid production. Based on the mathematical model predictions, growing algal cells in higher ammonium containing medium initially and switching to low ammonium containing medium in a later stage may result in elevated amounts of lipid production, which may be used for scale up and commercialization.

Original languageEnglish (US)
Pages (from-to)419-424
Number of pages6
JournalBioresource Technology
Volume119
DOIs
StatePublished - Sep 1 2012

Fingerprint

Ammonium Compounds
Lipids
ammonium
lipid
Cell growth
kinetics
Kinetics
modeling
Nitrogen
autotrophy
nitrogen
commercialization
Bioreactors
bioreactor
Sensitivity analysis
sensitivity analysis
Logistics
logistics
Phosphates
Carbon

Keywords

  • Autotrophy
  • Cell growth
  • Chlamydomonas reinhardtii
  • Mathematical model
  • Neutral lipid

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

Kinetic modeling of photoautotropic growth and neutral lipid accumulation in terms of ammonium concentration in Chlamydomonas reinhardtii. / Tevatia, Rahul; Demirel, Yaşar; Blum, Paul.

In: Bioresource Technology, Vol. 119, 01.09.2012, p. 419-424.

Research output: Contribution to journalArticle

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