KH-type splicing regulatory protein is regulated by nuclear factor-κB signaling to mediate innate immunity in Caco-2 cells infected by Salmonella enteritidis

Yuanyang Nie, Mei Cao, Daoyan Wu, Ningzhe Li, Jingshan Peng, Sijun Yi, Xiaofan Yang, Mao Zhang, Guoku Hu, Jian Zhao

Research output: Contribution to journalArticle

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Abstract

Salmonella enteritidis infection occurs in enterogenous diseases, such as gastroenteritis and parenteral focal infection, which often involve inflammation of intestinal epithelial cells. The nuclear factor kappa B (NF-κB) pathway participates in the innate immune response to many gram-negative pathogenic bacteria and initiates inflammation in epithelial cells. KH-type splicing regulatory protein (KSRP) is a multi-domain RNA-binding protein that recruits the exosome-containing mRNA degradation complex to mRNAs coding for inflammatory response factors. However, it remains unclear whether KSRP is regulated by NF-κB signaling pathway in response to S. enteritidis infection and affects the development of inflammation. Accordingly, in this study, we investigated the role of KSRP in mediating the response to S. enteritidis in Caco-2 cells. The data revealed that S. enteritidis infection decreased KSRP expression, which was suppressed by blocking the NF-κB pathway. Additionally, S. enteritidis infection significantly increased the expression of inducible nitric oxide synthase and cyclooxygenase-2. Overexpression of KSRP reduced the expression levels of inflammatory factors in Caco-2 cells. KSRP was regulated by the NF-κB signaling pathway and participated in mediating the innate immune response to S. enteritidis infection in Caco-2 cells, and KSRP acted as a negative regulator of inflammatory gene expression.

Original languageEnglish (US)
Pages (from-to)669-676
Number of pages8
JournalFolia Microbiologica
Volume63
Issue number6
DOIs
StatePublished - Nov 1 2018

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Protein Splicing
Salmonella enteritidis
Caco-2 Cells
Innate Immunity
Salmonella Infections
NF-kappa B
Inflammation
Epithelial Cells
Focal Infection
Exosomes
RNA Stability
Gastroenteritis
Nitric Oxide Synthase Type II
Cyclooxygenase 2
Regulator Genes
Gram-Negative Bacteria
Gene Expression
Messenger RNA

ASJC Scopus subject areas

  • Microbiology

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KH-type splicing regulatory protein is regulated by nuclear factor-κB signaling to mediate innate immunity in Caco-2 cells infected by Salmonella enteritidis. / Nie, Yuanyang; Cao, Mei; Wu, Daoyan; Li, Ningzhe; Peng, Jingshan; Yi, Sijun; Yang, Xiaofan; Zhang, Mao; Hu, Guoku; Zhao, Jian.

In: Folia Microbiologica, Vol. 63, No. 6, 01.11.2018, p. 669-676.

Research output: Contribution to journalArticle

Nie, Yuanyang ; Cao, Mei ; Wu, Daoyan ; Li, Ningzhe ; Peng, Jingshan ; Yi, Sijun ; Yang, Xiaofan ; Zhang, Mao ; Hu, Guoku ; Zhao, Jian. / KH-type splicing regulatory protein is regulated by nuclear factor-κB signaling to mediate innate immunity in Caco-2 cells infected by Salmonella enteritidis. In: Folia Microbiologica. 2018 ; Vol. 63, No. 6. pp. 669-676.
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