K4, K9 and K18 in human histone H3 are targets for biotinylation by biotinidase

Keyna Kobza, Gabriela Camporeale, Brian Rueckert, Alice Kueh, Jacob B. Griffin, Gautam Sarath, Janos Zempleni

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Abstract

Histones are modified post-translationally, e.g. by methylation of lysine and arginine residues, and by phosphorylation of serine residues. These modifications regulate processes such as gene expression, DNA repair, and mitosis and meiosis. Recently, evidence has been provided that histones are also modified by covalent binding of the vitamin biotin. The aims of this study were to identify biotinylation sites in histone H3, and to investigate the crosstalk among histone biotinylation, methylation and phosphorylation. Synthetic peptides based on the sequence of human histone H3 were used as substrates for enzymatic biotinylation by biotinidase; biotin in peptides was probed using streptavidin peroxidase. These studies provided evidence that K4, K9 and K18 in histone H3 are good targets for biotinylation; K14 and K23 are relatively poor targets. Antibodies were generated to histone H3, biotinylated either at K4, K9 or K18. These antibodies localized to nuclei in human placental cells in immunocytochemistry and immunoblotting experiments, suggesting that lysines in histone H3 are biot-inylated in vivo. Dimethylation of R2, R8 and R17 increased biotinylation of K4, K9 and K18, respectively, by biotinidase; phosphorylation of S10 abolished biotinylation of K9. These observations are consistent with cross-talk between biotinylation of histones and other known modifications of histones. We speculate that this crosstalk provides a link to known roles for biotin in gene expression and cell proliferation.

Original languageEnglish (US)
Pages (from-to)4249-4259
Number of pages11
JournalFEBS Journal
Volume272
Issue number16
DOIs
Publication statusPublished - Aug 1 2005

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Keywords

  • Biotin
  • Biotinidase
  • Histone H3
  • Lysine

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Kobza, K., Camporeale, G., Rueckert, B., Kueh, A., Griffin, J. B., Sarath, G., & Zempleni, J. (2005). K4, K9 and K18 in human histone H3 are targets for biotinylation by biotinidase. FEBS Journal, 272(16), 4249-4259. https://doi.org/10.1111/j.1742-4658.2005.04839.x