K activity of CCD principal cells from normal and DOCA-treated rabbits

Steven Claude Sansom, S. Agulian, S. Muto, V. Illig, G. Giebisch

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Abstract

We used liquid ion exchanger and conventional microelectrodes to evaluate the effects of mineralocorticoids on the intracellular K activity (a(i)(K)) and K transport properties of principal cells (PC) of isolated cortical collecting ducts (CCDs). Hoffman modulation optics and electrophysiological methods were used to identify PC. K activity was measured with two single-barreled electrodes. We found that a(i)(K) of PC from deoxycorticosterone acetate (DOCA)-treated rabbits (97.6 mM) was not different from controls (94.8 mM). The driving forces for K transport across the basolateral membrane favored cell to bath (reabsorption) in PCs from controls and bath to cell (secretion) in PCs from DOCA-treated rabbits. However, the driving force for K secretion across the apical membrane was not significantly different between the two groups. We used the intracellular a(i)(K)s and bath ion substitutions (gluconate for Cl and K for Na) to evaluate the effects of DOCA on the ion-selective properties of the basolateral membrane of PC. DOCA increased P(K)/P(Cl) from 0.33 to 0.89. Our conclusion was as follows: in PC of control rabbits K is above electrochemical equilibrium across the basolateral membrane. However, the basolateral K conductance is probably too small for significant K recycling. In PC of DOCA-treated rabbits the a(i)(K) is below electrochemical equilibrium across the basolateral membrane and the K conductance is increased. These effects enhance K secretion across this border while maintaining cell K constant.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume256
Issue number1
StatePublished - Jan 1 1989

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Desoxycorticosterone
Acetates
Rabbits
Baths
Ions
Membranes
Cell Membrane
Mineralocorticoids
Recycling
Microelectrodes
Electrodes

ASJC Scopus subject areas

  • Physiology

Cite this

K activity of CCD principal cells from normal and DOCA-treated rabbits. / Sansom, Steven Claude; Agulian, S.; Muto, S.; Illig, V.; Giebisch, G.

In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology, Vol. 256, No. 1, 01.01.1989.

Research output: Contribution to journalArticle

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AU - Giebisch, G.

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