Juxtamedullary microvascular responses to arginine vasopressin in rat kidney

Lisa M. Harrison-Bernard, Pamela K Carmines

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Experiments were performed to determine the site of arginine vasopressin (AVP)-induced vascular resistance adjustments that result in decreases in papillary blood flow. Simultaneous measurements of luminal diameter and centerline erythrocyte velocity allowed estimation of single-vessel blood flow in in vitro blood-perfused juxtamedullary nephrons. AVP (0.1-1,000 pM) caused concentration-dependent reductions in outer medullary descending vasa recta (OMDVR) blood flow (10 pM) without altering OMDVR diameter. Afferent arteriolar diameter was significantly decreased by 1 pM AVP, whereas arcuate artery diameter was decreased by 100 pM AVP. Increasing the concentration of AVP to 1,000 pM resulted in significant reductions of vessel diameter in interlobular arteries, distal afferent arterioles, and efferent arterioles. The effects of AVP to decrease afferent arteriolar diameter and blood flow were blocked in the presence of V1 receptor antagonist. These data indicate that afferent arterioles exhibit the greatest sensitivity to the vasoconstrictor effects of AVP, whereas OMDVR appear insensitive. We conclude that the change in OMDVR blood flow produced by AVP is not due to a direct effect of the peptide on this vascular segment but to its effect on upstream vessels.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume267
Issue number2
StatePublished - Aug 30 1994

Fingerprint

Arginine Vasopressin
Kidney
Rectum
Arterioles
Blood Vessels
Arteries
Vasopressin Receptors
Erythrocyte Indices
Nephrons
Vasoconstrictor Agents
Vascular Resistance
Peptides

Keywords

  • blood flow
  • erythrocyte velocity
  • medullary circulation
  • video microscopy

ASJC Scopus subject areas

  • Physiology

Cite this

Juxtamedullary microvascular responses to arginine vasopressin in rat kidney. / Harrison-Bernard, Lisa M.; Carmines, Pamela K.

In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology, Vol. 267, No. 2, 30.08.1994.

Research output: Contribution to journalArticle

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